Accelerative effect of topical Zataria multiflora essential oil against infected wound model by modulating inflammation, angiogenesis, and collagen biosynthesis

• Published in: Pharmaceutical biology 2021-01, Vol.59 (1), p.1-10
• Main Authors: Farahpour, Mohammad Reza, Sheikh, Sara, Kafshdooz, Elham, Sonboli, Ali
• Format: Article
• Language: English
• Subjects: Angiogenesis; Antimicrobial agents; antimicrobial properties; Antioxidants; Collagen; Essential oils; Fibroblast growth factor 2; Fibroblasts; Herbal medicine; IL-1β; Infected wound healing; Inflammation; inflammatory cytokines; Insulin-like growth factor I; Interleukin 10; mRNA; Mupirocin; Oils & fats; Ointments; thymol; Tumor necrosis factor-α; Vascular endothelial growth factor; Wound healing; Zataria multiflora
• ISSN: 1388-0209; 1744-5116
• DOI: 10.1080/13880209.2020.1861029

Zataria multiflora Boiss (Lamiaceae) essential oil (ZME) is believed to be a bactericide herbal medicine and might alleviate negative effects of infection. This study evaluates the effects of an ointment prepared from ZME (ZMEO) on infected wounds. A full-thickness excisional skin wound was surgically created in each mouse and inoculated with 5 × 10 7 suspension containing Pseudomonas aeruginosa and Staphylococcus aureus. The BALB/c mice (n = 72) were divided into four groups: (1) negative control that received base ointment (NCG), (2) positive control that daily received Mupirocin ® (MG), (3) therapeutic ointment containing 2% ZMEO and (4) therapeutic ointment containing 4% ZMEO, for 21 days. Wound contraction, total bacterial count, histopathological parameters, antioxidant activity, qRT-PCR analysis for expression of IL-1β, TNF-α, VEGF, IGF-1, TGF-β, IL-10, and FGF-2 mRNA levels were assessed on days 3, 7, and 14 following the wounding. Topical administration of ZMEO significantly decreased the total bacterial count and wound area and also expression of IL-1β and TNF-α compared to the control groups (p