Electro-deposited synthesis of tube-like Collagen-Chitosan Hydrogel and its biological performances

• Published in: Biomedical materials (Bristol) 2021-01
• Main Authors: Li, Ping, He, Lang, Liu, Xueling, Fan, Shengxuan, Yuan, Yifan, Zhang, Juntao, Wang, Haibo, Li, Sheng
• Format: Article
• Language: English
• ISSN: 1748-605X

Electro-deposition is a smart, safe and efficient method for biomaterial manufacturing. Collagen, as a functional protein with excellent biocompatibility and biosafety, is a promising candidate for tissue engineering and biomedical applications. However, there is few reports on electro-deposition of biomaterials using collagen without electrically or magnetically active nanoparticles. In this study, electro-deposition was employed to fabricate a tube-like collagen-chitosan hydrogel in mild environment, swiftly. FT-IR was employed to analyze the ingredient of the tube-like hydrogel. The result shown that the hydrogel contained both collagen and chitosan. The distribution and content of collagen in the hydrogel was further measured by hematoxylin-eosin staining method and hydroxyproline titration. Collagen distributed homogenously and its content was related to the initial ratio of collagen/chitosan. The tension resistance of the composite gel and the thermal stability of collagen in the composite were obviously enhanced that benefit by the dope of chitosan. Meanwhile, the tube-like hydrogel retain the good ability of promoting cell proliferation of collagen. This method will offer a convenient approach for the design and fabrication of collagen based material, which could effectively retain the bioactivity and biosafety of collagen and furnish a new way to enhance the stability of collagen and tensile strength of collagen based materials.