Bioinformatics analysis reveals the landscape of immune cell infiltration and immune-related pathways participating in the progression of carotid atherosclerotic plaques

Atherosclerosis is a systemic disease associated with inflammatory cell infiltration and activation of immune-related pathways. In our study, we aimed to uncover immune-related changes and explore novel immunological features in the development of carotid atherosclerotic plaques. First, we applied i...

Full description

Saved in:
Bibliographic Details
Published in:Artificial cells, nanomedicine, and biotechnology nanomedicine, and biotechnology, 2021-01, Vol.49 (1), p.96-107
Main Authors: Tan, Liao, Xu, Qian, Shi, Ruizheng, Zhang, Guogang
Format: Article
Language:English
Subjects:
Arteriosclerosis
Atherosclerosis
Bioinformatics
Bioinformatics analysis
carotid atherosclerotic plaques
CD4 antigen
Cell activation
Foxp3 protein
Gene expression
Gene Expression Omnibus
Gene set enrichment analysis
immune cell infiltration
Immune system
Immunological memory
Immunology
Infiltration
Lymphocytes
Lymphocytes T
Memory cells
Plaques
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Atherosclerosis is a systemic disease associated with inflammatory cell infiltration and activation of immune-related pathways. In our study, we aimed to uncover immune-related changes and explore novel immunological features in the development of carotid atherosclerotic plaques. First, we applied integrated bioinformatics methods, including CIBERSORT and gene set enrichment analysis (GSEA). The gene expression matrices GSE28829, GSE41571, and GSE43292 were obtained from the Gene Expression Omnibus (GEO) dataset. After a series of data pre-processing steps, the resulting combined expression matrices were analysed using the CIBERSORT, GSEA, and Cluster Profiler packages. After the comparison and analysis between the carotid atherosclerotic plaques in the early and advanced stages, we discovered that there is a higher percentage of activated memory CD4 T cells and a lower percentage of resting memory CD4 cells in advanced-stage plaques. Moreover, activation of memory CD4 T cells can promote the development of carotid atherosclerotic plaques. Additionally, FOXP3 + Treg cell maturation can also participate in the progression of carotid plaques.
ISSN:2169-1401
2169-141X
DOI:10.1080/21691401.2021.1873798