A new in vitro model applied 90 Y microspheres to study the effects of low dose beta radiation on colorectal cancer cell line in various oxygenation conditions

We propose a new in vitro model to assess the impact of Y-microspheres derived low-dose beta radiation on colorectal cancer cell line under various oxygenation conditions that mimic the tumor environment. Cancer cells (HCT116) proliferation was assessed using Alamar Blue (AB) assay after 48, 72, and...

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Bibliographic Details
Published in:Scientific reports 2021-02, Vol.11 (1), p.4472
Main Authors: Piasecki, Piotr, Majewska, Aleksandra, Narloch, Jerzy, Maciak, Maciej, Brodaczewska, Klaudia, Kuc, Michal, Was, Halina, Wierzbicki, Marek, Brzozowski, Krzysztof, Ziecina, Piotr, Mazurek, Andrzej, Dziuk, Miroslaw, Iller, Edward, Kieda, Claudine
Format: Article
Language:English
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Summary:We propose a new in vitro model to assess the impact of Y-microspheres derived low-dose beta radiation on colorectal cancer cell line under various oxygenation conditions that mimic the tumor environment. Cancer cells (HCT116) proliferation was assessed using Alamar Blue (AB) assay after 48, 72, and 96 h. FLUKA code assessed changes in cancer cell populations relative to the absorbed dose. In normoxia, mitochondrial activity measured by Alamar Blue after 48-72 h was significantly correlated with the number of microspheres (48 h: r = 0.87 and 72 h: r = 0.89, p 
ISSN:2045-2322
DOI:10.1038/s41598-021-84000-7