Hyperthermophilic methanogenic archaea act as high-pressure CH 4 cell factories

Bioprocesses converting carbon dioxide with molecular hydrogen to methane (CH ) are currently being developed to enable a transition to a renewable energy production system. In this study, we present a comprehensive physiological and biotechnological examination of 80 methanogenic archaea (methanoge...

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Bibliographic Details
Published in:Communications biology 2021-03, Vol.4 (1), p.289
Main Authors: Mauerhofer, Lisa-Maria, Zwirtmayr, Sara, Pappenreiter, Patricia, Bernacchi, Sébastien, Seifert, Arne H, Reischl, Barbara, Schmider, Tilman, Taubner, Ruth-Sophie, Paulik, Christian, Rittmann, Simon K-M R
Format: Article
Language:English
Subjects:
Amino Acid Motifs
High-Throughput Screening Assays
Industrial Microbiology
Kinetics
Membrane Glycoproteins - metabolism
Methane - metabolism
Methanocaldococcaceae - growth & development
Methanocaldococcaceae - metabolism
Methanocaldococcus - growth & development
Methanocaldococcus - metabolism
Oxidoreductases - metabolism
Pressure
Renewable Energy
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Summary:Bioprocesses converting carbon dioxide with molecular hydrogen to methane (CH ) are currently being developed to enable a transition to a renewable energy production system. In this study, we present a comprehensive physiological and biotechnological examination of 80 methanogenic archaea (methanogens) quantifying growth and CH production kinetics at hyperbaric pressures up to 50 bar with regard to media, macro-, and micro-nutrient supply, specific genomic features, and cell envelope architecture. Our analysis aimed to systematically prioritize high-pressure and high-performance methanogens. We found that the hyperthermophilic methanococci Methanotorris igneus and Methanocaldococcoccus jannaschii are high-pressure CH cell factories. Furthermore, our analysis revealed that high-performance methanogens are covered with an S-layer, and that they harbour the amino acid motif Tyr Gly Tyr in the alpha subunit of the methyl-coenzyme M reductase. Thus, high-pressure biological CH production in pure culture could provide a purposeful route for the transition to a carbon-neutral bioenergy sector.
ISSN:2399-3642