Monitoring of Chemical Markers in Extraction of Traditional Medicinal Plants (Piper Nigrum, Curcuma Longa) Using Insitu ReactIR

• Published in: Journal of AOAC International 2021-03
• Main Authors: Reddy, K Sateesh, Siva, Bandi, Reddy, S Divya, Kumar, K, Pratap, T V, Reddy, Konda Vidyasagar, Venkateswara Rao, B, Suresh Babu, K
• Format: Article
• Language: English
• ISSN: 1944-7922

The fingerprinting and quantification of marker compounds from medicinal plants is a domain of herbal industry for quality/quantity control parameters. The main objective of this study is application of insitu ReactIR technique for measuring the concentration of different components during the extraction process of different medicinal plants. In this study we have performed the extraction of of two-marker compounds viz. piperine from P. nigrum, curcumin from C. longa plants using various solvents (dichloromethane and methanol) and progress of extraction was monitored using an in situ FTIR probe instrument and an automated reactor. In this communication, using in situ ReactIR technique we developed a method which demonstrates the relative quantification of marker analytes, optimizes extraction time and type of solvents to be used for different analytes during the extraction process. To the best of our knowledge, this is the first report for relative quantification and structural information of marker compounds during the process of extraction using in situ FTIR. The present study highlights about the Real-time monitoring, insitu quantification and structural information of marker compounds during the process of extraction of medicinal plants using in situ FTIR.