Time Course of Skeletal Muscle miRNA Expression following Resistance, High-Intensity Interval, and Concurrent Exercise

• Published in: Medicine and science in sports and exercise 2021-02
• Main Authors: Telles, Guilherme Defante, Libardi, Cleiton Augusto, Conceição, Miguel Soares, Vechin, Felipe Cassaro, Lixandrão, Manoel Emílio, de Andrade, André Luís Lugnani, Guedes, Daniel Novais, Ugrinowitsch, Carlos, Camera, Donny Michael
• Format: Article
• Language: English
• ISSN: 0195-9131; 1530-0315
• DOI: 10.1249/MSS.0000000000002632

Exercise-induced microRNAs (miRNAs) expression has been implicated in the regulation of skeletal muscle plasticity. However, the specificity and acute time course in miRNA expression following divergent exercise modes are unknown. In a randomized cross-over design, we compared the acute expression profile of eight skeletal muscle miRNAs previously reported to be involved in skeletal muscle development, growth and maintenance following a bout of either resistance exercise (RE), high intensity interval exercise (HIIE) and concurrent resistance and high intensity interval exercises (CE). Nine untrained young men (23.9±2.8y, 70.1±14.9kg, 177.2±3.0cm, 41.4±5.2ml·kg-1·min-1) underwent a counter-balanced cross-over design in which they performed bouts of RE (2x10 repetitions maximum 45°Leg Press and Leg Extension exercises), HIEE (12x1 min sprints at VO2peak with 1min rest intervals between sprints) and CE (RE followed by HIIE), separated by one week. Vastus lateralis biopsies were harvested immediately before (Pre), and immediately (0h), 4h and 8h after each exercise bout. There were similar increases (main effect of time; P