Development of nanobody-based flow-through dot ELISA and lateral-flow immunoassay for rapid detection of 3-phenoxybenzoic acid

As a major metabolite of pyrethroid pesticides, 3-phenoxybenzoic acid (3-PBA) can be an indicator of health risk and human exposure assessment. Based on nanobodies (Nbs), we have developed a rapid flow-through dot enzyme linked immunosorbent assay (dot ELISA) and gold nanoparticle (GNP) lateral-flow...

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Bibliographic Details
Published in:Analytical methods 2021-04, Vol.13 (14), p.1757-1765
Main Authors: Zhang, Can, Wu, Xiaoxiao, Li, Dongyang, Hu, Jinnuo, Wan, Debin, Zhang, Zhen, Hammock, Bruce D
Format: Article
Language:English
Subjects:
Benzoates
Enzyme-Linked Immunosorbent Assay
Flow
Gold
Health risks
Humans
Immunoassay
Immunoassays
Liquid chromatography
Mass spectrometry
Mass spectroscopy
Metabolites
Metal Nanoparticles
Nanobodies
Nanoparticles
Pesticides
Phenoxybenzoic acid
Pyrethroids
Rapid flow
Water analysis
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Summary:As a major metabolite of pyrethroid pesticides, 3-phenoxybenzoic acid (3-PBA) can be an indicator of health risk and human exposure assessment. Based on nanobodies (Nbs), we have developed a rapid flow-through dot enzyme linked immunosorbent assay (dot ELISA) and gold nanoparticle (GNP) lateral-flow immunoassay for detecting 3-PBA. The limit of detection (LOD) values for detecting 3-PBA by flow-through dot ELISA and GNP lateral-flow immunoassay were 0.01 ng mL −1 and 0.1 ng mL −1 , respectively. The samples (urine and lake water) with and without 3-PBA were detected by both nanobody-based flow-through dot ELISA and GNP lateral-flow immunoassay, as well as liquid chromatography-mass spectrometry (LC-MS) for validation. There was good consistency between the results of the immunoassays. This demonstrated that the two developed nanobody-based immunoassays are suitable for rapid detection of 3-PBA. Flow-through dot ELISA and gold nanoparticle lateral-flow immunoassay based on nanobodies were developed for detecting 3-PBA. The sensitivity of nanobody-based flow-through dot ELISA is 10-fold higher than that of gold nanoparticle lateral-flow immunoassay.
ISSN:1759-9660
1759-9679
DOI:10.1039/d1ay00129a