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Sodium butyrate converts Caco-2 monolayers into a leaky but healthy intestinal barrier resembling that of a newborn infant
A simple and reliable in vitro model of the infant intestinal barrier is needed to study nutrient absorption and drug permeability specifically for this life stage. This study investigated the treatment of 20 day old differentiated Caco-2 monolayers with sodium butyrate at various concentrations (0-...
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Published in: | Food & function 2021-06, Vol.12 (11), p.566-576 |
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creator | Kondrashina, Alina Brodkorb, Andre Giblin, Linda |
description | A simple and reliable
in vitro
model of the infant intestinal barrier is needed to study nutrient absorption and drug permeability specifically for this life stage. This study investigated the treatment of 20 day old differentiated Caco-2 monolayers with sodium butyrate at various concentrations (0-250 mM). Monolayer integrity, cytotoxicity, permeability and inflammatory response were tracked. An intestinal barrier model, with infant gut characteristics, was developed based on the treatment of mature monolayers with 125 mM sodium butyrate for 24 h. Such treatment was not cytotoxic but caused a stable transepithelial electrical resistance value of 408 ± 52 Ω cm
2
. The ratio of lactulose to mannitol transport across the intestinal barrier increased 1.79-fold. Redistribution of the tight junction proteins, occludin and ZO-1, in response to sodium butyrate treatment was visualized with immunofluorescence. Levels of the cytokines, TNF-α and IL-6, although modestly increased did not indicate an inflammatory response by Caco-2 to sodium butyrate. This intestinal barrier demonstrated physiologically relevant transport rates for dairy protein of 0.01-0.06%, suggesting it may be used to track permeability of proteins in infant nutritional products.
Treating Caco2 monolayers with sodium butyrate will create,
in vitro
, a leaky but healthy gut barrier that closely resembles that of a newborn baby. |
doi_str_mv | 10.1039/d1fo00519g |
format | article |
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in vitro
model of the infant intestinal barrier is needed to study nutrient absorption and drug permeability specifically for this life stage. This study investigated the treatment of 20 day old differentiated Caco-2 monolayers with sodium butyrate at various concentrations (0-250 mM). Monolayer integrity, cytotoxicity, permeability and inflammatory response were tracked. An intestinal barrier model, with infant gut characteristics, was developed based on the treatment of mature monolayers with 125 mM sodium butyrate for 24 h. Such treatment was not cytotoxic but caused a stable transepithelial electrical resistance value of 408 ± 52 Ω cm
2
. The ratio of lactulose to mannitol transport across the intestinal barrier increased 1.79-fold. Redistribution of the tight junction proteins, occludin and ZO-1, in response to sodium butyrate treatment was visualized with immunofluorescence. Levels of the cytokines, TNF-α and IL-6, although modestly increased did not indicate an inflammatory response by Caco-2 to sodium butyrate. This intestinal barrier demonstrated physiologically relevant transport rates for dairy protein of 0.01-0.06%, suggesting it may be used to track permeability of proteins in infant nutritional products.
Treating Caco2 monolayers with sodium butyrate will create,
in vitro
, a leaky but healthy gut barrier that closely resembles that of a newborn baby.</description><identifier>ISSN: 2042-6496</identifier><identifier>EISSN: 2042-650X</identifier><identifier>DOI: 10.1039/d1fo00519g</identifier><identifier>PMID: 33960994</identifier><language>eng</language><publisher>England: Royal Society of Chemistry</publisher><subject>Cytokines ; Cytotoxicity ; Electrical resistivity ; Immunofluorescence ; Infants ; Inflammation ; Inflammatory response ; Interleukin 6 ; Intestine ; Lactulose ; Mannitol ; Membrane permeability ; Monolayers ; Permeability ; Protein transport ; Proteins ; Sodium ; Sodium butyrate ; Toxicity ; Tumor necrosis factor-α ; Zonula occludens-1 protein</subject><ispartof>Food & function, 2021-06, Vol.12 (11), p.566-576</ispartof><rights>Copyright Royal Society of Chemistry 2021</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c337t-b04012feb5cfcb56a62d160dbb3ce86a446b0b4e1340ae7686652fe5ec7bf84b3</citedby><cites>FETCH-LOGICAL-c337t-b04012feb5cfcb56a62d160dbb3ce86a446b0b4e1340ae7686652fe5ec7bf84b3</cites><orcidid>0000-0002-9354-3121</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/33960994$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kondrashina, Alina</creatorcontrib><creatorcontrib>Brodkorb, Andre</creatorcontrib><creatorcontrib>Giblin, Linda</creatorcontrib><title>Sodium butyrate converts Caco-2 monolayers into a leaky but healthy intestinal barrier resembling that of a newborn infant</title><title>Food & function</title><addtitle>Food Funct</addtitle><description>A simple and reliable
in vitro
model of the infant intestinal barrier is needed to study nutrient absorption and drug permeability specifically for this life stage. This study investigated the treatment of 20 day old differentiated Caco-2 monolayers with sodium butyrate at various concentrations (0-250 mM). Monolayer integrity, cytotoxicity, permeability and inflammatory response were tracked. An intestinal barrier model, with infant gut characteristics, was developed based on the treatment of mature monolayers with 125 mM sodium butyrate for 24 h. Such treatment was not cytotoxic but caused a stable transepithelial electrical resistance value of 408 ± 52 Ω cm
2
. The ratio of lactulose to mannitol transport across the intestinal barrier increased 1.79-fold. Redistribution of the tight junction proteins, occludin and ZO-1, in response to sodium butyrate treatment was visualized with immunofluorescence. Levels of the cytokines, TNF-α and IL-6, although modestly increased did not indicate an inflammatory response by Caco-2 to sodium butyrate. This intestinal barrier demonstrated physiologically relevant transport rates for dairy protein of 0.01-0.06%, suggesting it may be used to track permeability of proteins in infant nutritional products.
Treating Caco2 monolayers with sodium butyrate will create,
in vitro
, a leaky but healthy gut barrier that closely resembles that of a newborn baby.</description><subject>Cytokines</subject><subject>Cytotoxicity</subject><subject>Electrical resistivity</subject><subject>Immunofluorescence</subject><subject>Infants</subject><subject>Inflammation</subject><subject>Inflammatory response</subject><subject>Interleukin 6</subject><subject>Intestine</subject><subject>Lactulose</subject><subject>Mannitol</subject><subject>Membrane permeability</subject><subject>Monolayers</subject><subject>Permeability</subject><subject>Protein transport</subject><subject>Proteins</subject><subject>Sodium</subject><subject>Sodium butyrate</subject><subject>Toxicity</subject><subject>Tumor necrosis factor-α</subject><subject>Zonula occludens-1 protein</subject><issn>2042-6496</issn><issn>2042-650X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><recordid>eNpd0c9r2zAUB3AxWtbQ5rL7hqCXMnAnWbJsHUu6_oBCDluhNyPJz407W0olucP966ssaQrTRYL3eQ9JX4S-UHJOCZM_Gto6QgoqHz-hWU54nomCPBy8n7kUR2gewhNJi0lZyeozOmJMCiIln6HXX67pxgHrMU5eRcDG2RfwMeCFMi7L8eCs69UEPuDORocV7kH9mTYNeAWqj6tpU4AQO6t6rJX3HXjsIcCg-84-4rhSEbs2dVr4q523ybfKxhN02Ko-wHy3H6P7q5-_FzfZ3fL6dnFxlxnGyphpwgnNW9CFaY0uhBJ5QwVptGYGKqE4F5poDpRxoqAUlRBF4gWYUrcV1-wYnW3nrr17HtNF66ELBvpeWXBjqPMi56wocyITPf2PPrnRp3dtFCslS59aJfV9q4x3IXho67XvBuWnmpJ6E0p9Sa-W_0K5TvjbbuSoB2j29D2CBL5ugQ9mX_1Ilb0BWxKSag</recordid><startdate>20210608</startdate><enddate>20210608</enddate><creator>Kondrashina, Alina</creator><creator>Brodkorb, Andre</creator><creator>Giblin, Linda</creator><general>Royal Society of Chemistry</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>7T7</scope><scope>7TO</scope><scope>7U7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-9354-3121</orcidid></search><sort><creationdate>20210608</creationdate><title>Sodium butyrate converts Caco-2 monolayers into a leaky but healthy intestinal barrier resembling that of a newborn infant</title><author>Kondrashina, Alina ; Brodkorb, Andre ; Giblin, Linda</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c337t-b04012feb5cfcb56a62d160dbb3ce86a446b0b4e1340ae7686652fe5ec7bf84b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Cytokines</topic><topic>Cytotoxicity</topic><topic>Electrical resistivity</topic><topic>Immunofluorescence</topic><topic>Infants</topic><topic>Inflammation</topic><topic>Inflammatory response</topic><topic>Interleukin 6</topic><topic>Intestine</topic><topic>Lactulose</topic><topic>Mannitol</topic><topic>Membrane permeability</topic><topic>Monolayers</topic><topic>Permeability</topic><topic>Protein transport</topic><topic>Proteins</topic><topic>Sodium</topic><topic>Sodium butyrate</topic><topic>Toxicity</topic><topic>Tumor necrosis factor-α</topic><topic>Zonula occludens-1 protein</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kondrashina, Alina</creatorcontrib><creatorcontrib>Brodkorb, Andre</creatorcontrib><creatorcontrib>Giblin, Linda</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Food & function</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kondrashina, Alina</au><au>Brodkorb, Andre</au><au>Giblin, Linda</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Sodium butyrate converts Caco-2 monolayers into a leaky but healthy intestinal barrier resembling that of a newborn infant</atitle><jtitle>Food & function</jtitle><addtitle>Food Funct</addtitle><date>2021-06-08</date><risdate>2021</risdate><volume>12</volume><issue>11</issue><spage>566</spage><epage>576</epage><pages>566-576</pages><issn>2042-6496</issn><eissn>2042-650X</eissn><abstract>A simple and reliable
in vitro
model of the infant intestinal barrier is needed to study nutrient absorption and drug permeability specifically for this life stage. This study investigated the treatment of 20 day old differentiated Caco-2 monolayers with sodium butyrate at various concentrations (0-250 mM). Monolayer integrity, cytotoxicity, permeability and inflammatory response were tracked. An intestinal barrier model, with infant gut characteristics, was developed based on the treatment of mature monolayers with 125 mM sodium butyrate for 24 h. Such treatment was not cytotoxic but caused a stable transepithelial electrical resistance value of 408 ± 52 Ω cm
2
. The ratio of lactulose to mannitol transport across the intestinal barrier increased 1.79-fold. Redistribution of the tight junction proteins, occludin and ZO-1, in response to sodium butyrate treatment was visualized with immunofluorescence. Levels of the cytokines, TNF-α and IL-6, although modestly increased did not indicate an inflammatory response by Caco-2 to sodium butyrate. This intestinal barrier demonstrated physiologically relevant transport rates for dairy protein of 0.01-0.06%, suggesting it may be used to track permeability of proteins in infant nutritional products.
Treating Caco2 monolayers with sodium butyrate will create,
in vitro
, a leaky but healthy gut barrier that closely resembles that of a newborn baby.</abstract><cop>England</cop><pub>Royal Society of Chemistry</pub><pmid>33960994</pmid><doi>10.1039/d1fo00519g</doi><tpages>11</tpages><orcidid>https://orcid.org/0000-0002-9354-3121</orcidid></addata></record> |
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source | Royal Society of Chemistry:Jisc Collections:Royal Society of Chemistry Read and Publish 2022-2024 (reading list) |
subjects | Cytokines Cytotoxicity Electrical resistivity Immunofluorescence Infants Inflammation Inflammatory response Interleukin 6 Intestine Lactulose Mannitol Membrane permeability Monolayers Permeability Protein transport Proteins Sodium Sodium butyrate Toxicity Tumor necrosis factor-α Zonula occludens-1 protein |
title | Sodium butyrate converts Caco-2 monolayers into a leaky but healthy intestinal barrier resembling that of a newborn infant |
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