PVT 1 Promoted the Proliferation and Migration Ability of BMSCs in Glioma C6 Microenvironment

To investigate the changes in the proliferation and migration ability of bone marrow mesenchymal stem cells (BMSCs) after indirect co-culturing with glioma C6 cells, and to examine the role of plasmacytoma variant translocation 1 gene ( 1), a long non-coding RNA (lncRNA), in these changes. After sep...

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Bibliographic Details
Published in:Sichuan da xue xue bao. Journal of Sichuan University. Yi xue ban 2021-05, Vol.52 (3), p.438
Main Authors: Liu, Dong-Rong, Liu, Yan, Wu, Pei-Lian, Zheng, Lei-Lei, Huang, Xiao-Ya, Meng, Xue-Huan, Hu, Yun
Format: Article
Language:Chinese
Subjects:
Cell Line, Tumor
Cell Movement
Cell Proliferation
Glioma - genetics
Humans
Mesenchymal Stem Cells
RNA, Long Noncoding - genetics
Tumor Microenvironment
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Summary:To investigate the changes in the proliferation and migration ability of bone marrow mesenchymal stem cells (BMSCs) after indirect co-culturing with glioma C6 cells, and to examine the role of plasmacytoma variant translocation 1 gene ( 1), a long non-coding RNA (lncRNA), in these changes. After separation, cultivation and identification of BMSCs, BMSCs of good growth condition were picked out and indirectly co-cultured with glioma C6 cells in Transwell chambers. These cells are henceforth referred to as the co-culture group. Normal BMSCs cultured separately were the control group. CCK-8 and soft agar colony formation assay were used to examine the proliferation ability of the two groups of cells. Flow cytometry was used to examine the cell cycle. Wound healing assay and Transwell assay were used to explore the migration ability of the cells. Quantitative real-time PCR (qRT-PCR) was used to examine the genetic expression level of 1 in the two groups. The above-mentioned tests were repeated after the co-cultur
ISSN:1672-173X
DOI:10.12182/20210560203