Comparison of 1 H-MRS, Dixon fat-water separation and Z-spectral imaging for quantification of brown adipose tissue in rats

• Published in: Nan fang yi ke da xue xue bao = Journal of Southern Medical University 2021-05, Vol.41 (5), p.783
• Main Authors: Cai, Z, Yi, P, Tao, Q, Feng, Y
• Format: Article
• Language: Chinese
• Subjects: Adipose Tissue - diagnostic imaging; Adipose Tissue, Brown - diagnostic imaging; Animals; Magnetic Resonance Imaging; Magnetic Resonance Spectroscopy; Proton Magnetic Resonance Spectroscopy; Rats; Water
• ISSN: 1673-4254
• DOI: 10.12122/j.issn.1673-4254.2021.05.21

To evaluate the performance of H-magnetic resonance spectroscopy ( H-MRS), Dixon fat-water separation and Z-spectral magnetic resonance imaging (ZS-MRI) for quantification of fat content in phantoms and brown adipose tissues in rats. First, six water-oil mixture phantoms with different fat fractions (0, 20%, 40%, 60%, 80% and 100%) were prepared and placed in a 50-mL centrifuge tube. ZS-MRI, H-MRS and Dixon's method were used to quantitatively evaluate the fat content of the phantom, and the results were compared against the actual fat fractions. Then, ZS-MRI and Dixon's method were used to collect the data in the interscapular region of 6 rats, the fat-water distribution map was calculated, and the results were compared with H-MRS. ZS-MRI accurately quantified fat contents in the phantoms (Y=0.95*X+1.48). ZS-MRI was capable of distinguishing brown adipose tissue from white adipose tissue and defining the spatial distribution of the adipose tissue, and the results were highly consistent with those obtained by