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Protective effect of Phaeoporus obliquus polysaccharide against acute liver injury induced by carbon tetrachloride and alcohol in mice
Studied the optimum extraction process of polysaccharide from Phaeoporus obliquus and the effect of Phaeoporus obliquus polysaccharide on carbon tetrachloride (CCl )- or alcohol-induced acute liver injury in mice. The main factor in influencing the extraction rate of Phaeoporus obliquus polysacchari...
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| Published in: | Pakistan journal of pharmaceutical sciences 2021-03, Vol.34 (2), p.649 |
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| Main Authors: | , , , , , , , , |
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| container_issue | 2 |
| container_start_page | 649 |
| container_title | Pakistan journal of pharmaceutical sciences |
| container_volume | 34 |
| creator | Lei, Zhang Xiao-Hong, Ren Jing, He Wan-Nan, Li Jing-Wei, Hao Yun-Rong, Jing Jun-Rong, Chai Xiao-Wei, Sun Chun-Lei, Wan |
| description | Studied the optimum extraction process of polysaccharide from Phaeoporus obliquus and the effect of Phaeoporus obliquus polysaccharide on carbon tetrachloride (CCl
)- or alcohol-induced acute liver injury in mice. The main factor in influencing the extraction rate of Phaeoporus obliquus polysaccharide were extraction power and time, which was a kind of pyran glucose by infrared spectroscopy. CCl
and alcohol were employed respectively to establish CCl4 and alcohol-induced acute liver injury mouse models. Compared with model groups mice, Phaeoporus obliquus polysaccharide treatment at the doses of 100mg/kg and 200mg/kg exhibited an obvious reduction liver index, ALP, ALT, AST levels, MDA content and TNF-α level (p |
| doi_str_mv | 10.36721/PJPS.2021.34.2.REG.649-656.1 |
| format | article |
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)- or alcohol-induced acute liver injury in mice. The main factor in influencing the extraction rate of Phaeoporus obliquus polysaccharide were extraction power and time, which was a kind of pyran glucose by infrared spectroscopy. CCl
and alcohol were employed respectively to establish CCl4 and alcohol-induced acute liver injury mouse models. Compared with model groups mice, Phaeoporus obliquus polysaccharide treatment at the doses of 100mg/kg and 200mg/kg exhibited an obvious reduction liver index, ALP, ALT, AST levels, MDA content and TNF-α level (p<0.01) and SOD activity was increased, which was in a dose-dependent manner. Compared with the model group, the necrosis degree of hepatocytes was obviously reduced and the small fat droplets were formed in some cytoplasm, especially in high dose group, which the liver cells recovered to the level of normal group. Rt-PCR results showed that the expression of CYP2E1 mRNA in liver tissues of Phaeoporus obliquus polysaccharide groups were significantly reduced, and the difference were statistically significant compared with the model group (p<0.05). These results demonstrated that Phaeoporus obliquus polysaccharide has significantly hepatoprotective effect on CCl4 and alcohol-induced acute liver injury in mice.</description><identifier>ISSN: 1011-601X</identifier><identifier>DOI: 10.36721/PJPS.2021.34.2.REG.649-656.1</identifier><identifier>PMID: 34275842</identifier><language>eng</language><publisher>Pakistan</publisher><subject>Alanine Transaminase - drug effects ; Alanine Transaminase - metabolism ; Alkaline Phosphatase - drug effects ; Alkaline Phosphatase - metabolism ; Animals ; Aspartate Aminotransferases - drug effects ; Aspartate Aminotransferases - metabolism ; Carbon Tetrachloride - toxicity ; Central Nervous System Depressants - toxicity ; Chemical and Drug Induced Liver Injury - metabolism ; Cytochrome P-450 CYP2E1 - drug effects ; Cytochrome P-450 CYP2E1 - genetics ; Ethanol - toxicity ; Fungal Polysaccharides - pharmacology ; Hepatocytes - drug effects ; Hepatocytes - metabolism ; Hepatocytes - pathology ; Inonotus ; Liver - drug effects ; Liver - metabolism ; Liver - pathology ; Liver Diseases, Alcoholic - metabolism ; Malondialdehyde - metabolism ; Mice ; RNA, Messenger - drug effects ; RNA, Messenger - metabolism ; Superoxide Dismutase - drug effects ; Superoxide Dismutase - metabolism ; Tumor Necrosis Factor-alpha - drug effects ; Tumor Necrosis Factor-alpha - metabolism</subject><ispartof>Pakistan journal of pharmaceutical sciences, 2021-03, Vol.34 (2), p.649</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c238t-73e9dd9380d7ca0cc4140ea06d8ea0b510fe8cdc3a0d2de25edfd75d858703723</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/34275842$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lei, Zhang</creatorcontrib><creatorcontrib>Xiao-Hong, Ren</creatorcontrib><creatorcontrib>Jing, He</creatorcontrib><creatorcontrib>Wan-Nan, Li</creatorcontrib><creatorcontrib>Jing-Wei, Hao</creatorcontrib><creatorcontrib>Yun-Rong, Jing</creatorcontrib><creatorcontrib>Jun-Rong, Chai</creatorcontrib><creatorcontrib>Xiao-Wei, Sun</creatorcontrib><creatorcontrib>Chun-Lei, Wan</creatorcontrib><title>Protective effect of Phaeoporus obliquus polysaccharide against acute liver injury induced by carbon tetrachloride and alcohol in mice</title><title>Pakistan journal of pharmaceutical sciences</title><addtitle>Pak J Pharm Sci</addtitle><description>Studied the optimum extraction process of polysaccharide from Phaeoporus obliquus and the effect of Phaeoporus obliquus polysaccharide on carbon tetrachloride (CCl
)- or alcohol-induced acute liver injury in mice. The main factor in influencing the extraction rate of Phaeoporus obliquus polysaccharide were extraction power and time, which was a kind of pyran glucose by infrared spectroscopy. CCl
and alcohol were employed respectively to establish CCl4 and alcohol-induced acute liver injury mouse models. Compared with model groups mice, Phaeoporus obliquus polysaccharide treatment at the doses of 100mg/kg and 200mg/kg exhibited an obvious reduction liver index, ALP, ALT, AST levels, MDA content and TNF-α level (p<0.01) and SOD activity was increased, which was in a dose-dependent manner. Compared with the model group, the necrosis degree of hepatocytes was obviously reduced and the small fat droplets were formed in some cytoplasm, especially in high dose group, which the liver cells recovered to the level of normal group. Rt-PCR results showed that the expression of CYP2E1 mRNA in liver tissues of Phaeoporus obliquus polysaccharide groups were significantly reduced, and the difference were statistically significant compared with the model group (p<0.05). These results demonstrated that Phaeoporus obliquus polysaccharide has significantly hepatoprotective effect on CCl4 and alcohol-induced acute liver injury in mice.</description><subject>Alanine Transaminase - drug effects</subject><subject>Alanine Transaminase - metabolism</subject><subject>Alkaline Phosphatase - drug effects</subject><subject>Alkaline Phosphatase - metabolism</subject><subject>Animals</subject><subject>Aspartate Aminotransferases - drug effects</subject><subject>Aspartate Aminotransferases - metabolism</subject><subject>Carbon Tetrachloride - toxicity</subject><subject>Central Nervous System Depressants - toxicity</subject><subject>Chemical and Drug Induced Liver Injury - metabolism</subject><subject>Cytochrome P-450 CYP2E1 - drug effects</subject><subject>Cytochrome P-450 CYP2E1 - genetics</subject><subject>Ethanol - toxicity</subject><subject>Fungal Polysaccharides - pharmacology</subject><subject>Hepatocytes - drug effects</subject><subject>Hepatocytes - metabolism</subject><subject>Hepatocytes - pathology</subject><subject>Inonotus</subject><subject>Liver - drug effects</subject><subject>Liver - metabolism</subject><subject>Liver - pathology</subject><subject>Liver Diseases, Alcoholic - metabolism</subject><subject>Malondialdehyde - metabolism</subject><subject>Mice</subject><subject>RNA, Messenger - drug effects</subject><subject>RNA, Messenger - metabolism</subject><subject>Superoxide Dismutase - drug effects</subject><subject>Superoxide Dismutase - metabolism</subject><subject>Tumor Necrosis Factor-alpha - drug effects</subject><subject>Tumor Necrosis Factor-alpha - metabolism</subject><issn>1011-601X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><recordid>eNo1UMlKxEAU7IPijKO_IH3xmLaXrEcZxo0Bgwt4GzrvvZgMmXTsJEJ-wO82MHqpKqjlUIxdKylMnGh1kz_lr0JLrYQJhRYvm3sRh1kQR7FQJ2yppFJBLNXHgp33_V7K2cyyM7YwoU6iNNRL9pN7NxAM9TdxKstZcVfyvLLkOufHnruiqb_GWXSumXoLUFlfI3H7aeu2H7iFcSDezH3P63Y_-mkmHIGQFxMH6wvX8oEGb6Fq3LHaIrcNuMo1c5YfaqALdlrapqfLP16x97vN2_oh2D7fP65vtwFokw5BYihDzEwqMQErAUIVSrIyxnTGIlKypBQQjJWokXREWGISYRqliTSJNit2ddztxuJAuOt8fbB-2v0fYn4BDkRoHQ</recordid><startdate>202103</startdate><enddate>202103</enddate><creator>Lei, Zhang</creator><creator>Xiao-Hong, Ren</creator><creator>Jing, He</creator><creator>Wan-Nan, Li</creator><creator>Jing-Wei, Hao</creator><creator>Yun-Rong, Jing</creator><creator>Jun-Rong, Chai</creator><creator>Xiao-Wei, Sun</creator><creator>Chun-Lei, Wan</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope></search><sort><creationdate>202103</creationdate><title>Protective effect of Phaeoporus obliquus polysaccharide against acute liver injury induced by carbon tetrachloride and alcohol in mice</title><author>Lei, Zhang ; Xiao-Hong, Ren ; Jing, He ; Wan-Nan, Li ; Jing-Wei, Hao ; Yun-Rong, Jing ; Jun-Rong, Chai ; Xiao-Wei, Sun ; Chun-Lei, Wan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c238t-73e9dd9380d7ca0cc4140ea06d8ea0b510fe8cdc3a0d2de25edfd75d858703723</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Alanine Transaminase - drug effects</topic><topic>Alanine Transaminase - metabolism</topic><topic>Alkaline Phosphatase - drug effects</topic><topic>Alkaline Phosphatase - metabolism</topic><topic>Animals</topic><topic>Aspartate Aminotransferases - drug effects</topic><topic>Aspartate Aminotransferases - metabolism</topic><topic>Carbon Tetrachloride - toxicity</topic><topic>Central Nervous System Depressants - toxicity</topic><topic>Chemical and Drug Induced Liver Injury - metabolism</topic><topic>Cytochrome P-450 CYP2E1 - drug effects</topic><topic>Cytochrome P-450 CYP2E1 - genetics</topic><topic>Ethanol - toxicity</topic><topic>Fungal Polysaccharides - pharmacology</topic><topic>Hepatocytes - drug effects</topic><topic>Hepatocytes - metabolism</topic><topic>Hepatocytes - pathology</topic><topic>Inonotus</topic><topic>Liver - drug effects</topic><topic>Liver - metabolism</topic><topic>Liver - pathology</topic><topic>Liver Diseases, Alcoholic - metabolism</topic><topic>Malondialdehyde - metabolism</topic><topic>Mice</topic><topic>RNA, Messenger - drug effects</topic><topic>RNA, Messenger - metabolism</topic><topic>Superoxide Dismutase - drug effects</topic><topic>Superoxide Dismutase - metabolism</topic><topic>Tumor Necrosis Factor-alpha - drug effects</topic><topic>Tumor Necrosis Factor-alpha - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lei, Zhang</creatorcontrib><creatorcontrib>Xiao-Hong, Ren</creatorcontrib><creatorcontrib>Jing, He</creatorcontrib><creatorcontrib>Wan-Nan, Li</creatorcontrib><creatorcontrib>Jing-Wei, Hao</creatorcontrib><creatorcontrib>Yun-Rong, Jing</creatorcontrib><creatorcontrib>Jun-Rong, Chai</creatorcontrib><creatorcontrib>Xiao-Wei, Sun</creatorcontrib><creatorcontrib>Chun-Lei, Wan</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><jtitle>Pakistan journal of pharmaceutical sciences</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lei, Zhang</au><au>Xiao-Hong, Ren</au><au>Jing, He</au><au>Wan-Nan, Li</au><au>Jing-Wei, Hao</au><au>Yun-Rong, Jing</au><au>Jun-Rong, Chai</au><au>Xiao-Wei, Sun</au><au>Chun-Lei, Wan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Protective effect of Phaeoporus obliquus polysaccharide against acute liver injury induced by carbon tetrachloride and alcohol in mice</atitle><jtitle>Pakistan journal of pharmaceutical sciences</jtitle><addtitle>Pak J Pharm Sci</addtitle><date>2021-03</date><risdate>2021</risdate><volume>34</volume><issue>2</issue><spage>649</spage><pages>649-</pages><issn>1011-601X</issn><abstract>Studied the optimum extraction process of polysaccharide from Phaeoporus obliquus and the effect of Phaeoporus obliquus polysaccharide on carbon tetrachloride (CCl
)- or alcohol-induced acute liver injury in mice. The main factor in influencing the extraction rate of Phaeoporus obliquus polysaccharide were extraction power and time, which was a kind of pyran glucose by infrared spectroscopy. CCl
and alcohol were employed respectively to establish CCl4 and alcohol-induced acute liver injury mouse models. Compared with model groups mice, Phaeoporus obliquus polysaccharide treatment at the doses of 100mg/kg and 200mg/kg exhibited an obvious reduction liver index, ALP, ALT, AST levels, MDA content and TNF-α level (p<0.01) and SOD activity was increased, which was in a dose-dependent manner. Compared with the model group, the necrosis degree of hepatocytes was obviously reduced and the small fat droplets were formed in some cytoplasm, especially in high dose group, which the liver cells recovered to the level of normal group. Rt-PCR results showed that the expression of CYP2E1 mRNA in liver tissues of Phaeoporus obliquus polysaccharide groups were significantly reduced, and the difference were statistically significant compared with the model group (p<0.05). These results demonstrated that Phaeoporus obliquus polysaccharide has significantly hepatoprotective effect on CCl4 and alcohol-induced acute liver injury in mice.</abstract><cop>Pakistan</cop><pmid>34275842</pmid><doi>10.36721/PJPS.2021.34.2.REG.649-656.1</doi></addata></record> |
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| ispartof | Pakistan journal of pharmaceutical sciences, 2021-03, Vol.34 (2), p.649 |
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| language | eng |
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| source | Electronic Journals Library |
| subjects | Alanine Transaminase - drug effects Alanine Transaminase - metabolism Alkaline Phosphatase - drug effects Alkaline Phosphatase - metabolism Animals Aspartate Aminotransferases - drug effects Aspartate Aminotransferases - metabolism Carbon Tetrachloride - toxicity Central Nervous System Depressants - toxicity Chemical and Drug Induced Liver Injury - metabolism Cytochrome P-450 CYP2E1 - drug effects Cytochrome P-450 CYP2E1 - genetics Ethanol - toxicity Fungal Polysaccharides - pharmacology Hepatocytes - drug effects Hepatocytes - metabolism Hepatocytes - pathology Inonotus Liver - drug effects Liver - metabolism Liver - pathology Liver Diseases, Alcoholic - metabolism Malondialdehyde - metabolism Mice RNA, Messenger - drug effects RNA, Messenger - metabolism Superoxide Dismutase - drug effects Superoxide Dismutase - metabolism Tumor Necrosis Factor-alpha - drug effects Tumor Necrosis Factor-alpha - metabolism |
| title | Protective effect of Phaeoporus obliquus polysaccharide against acute liver injury induced by carbon tetrachloride and alcohol in mice |
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