Influence of altered transcription on the translational control of human ferritin expression

In this paper, we examine the response of a translational regulatory mechanism when changes in mRNA levels are induced. The gene that encodes the human ferritin heavy chain has been transfected into mouse fibroblasts. Stable transformants that express the human ferritin heavy chain have been isolate...

Full description

Saved in:
Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1987-09, Vol.84 (18), p.6335-6339
Main Authors: Rouault, T.A, Hentze, M.W, Dancis, A, Caughman, W, Harford, J.B, Klausner, R.D
Format: Article
Language:English
Subjects:
Biological and medical sciences
BIOSINTESIS
BIOSYNTHESE
BIOSYNTHESIS
Butyrates
Butyrates - pharmacology
Butyric Acid
Cell lines
Cellular metabolism
CONTROL GENETICO
FER
Ferritins
Ferritins - genetics
Fibroblasts
Fundamental and applied biological sciences. Psychology
GENE
Gene expression
Gene expression regulation
Gene Expression Regulation - drug effects
GENERO HUMANO
GENES
GENETIC CONTROL
GENRE HUMAIN
HIERRO
Humans
IRON
K562 cells
LUTTE GENETIQUE
MANKIND
Messenger RNA
METABOLISME DES MINERAUX
METABOLISMO DE MINERALES
METALLOPROTEINE
METALLOPROTEINS
METALPROTEINAS
MICE
MINERAL METABOLISM
Molecular and cellular biology
Molecular genetics
Protein Biosynthesis
RATON
RNA
SOURIS
Transcription, Genetic
Transfection
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:In this paper, we examine the response of a translational regulatory mechanism when changes in mRNA levels are induced. The gene that encodes the human ferritin heavy chain has been transfected into mouse fibroblasts. Stable transformants that express the human ferritin heavy chain have been isolated. This protein assembles into ferritin polymers and can co-assemble with host mouse ferritin. Biosynthetic rates of the expressed human ferritin varied over a wide range in response to perturbations in iron supply, but total and cytoplasmic messenger RNA levels remained unchanged. When changes in ferritin mRNA levels were induced by treatment with sodium butyrate, proportional changes in the biosynthetic rates of ferritin were observed, but the capacity for modulating biosynthesis in response to alterations in iron availability was preserved. These findings suggest that the final protein biosynthetic rate of a translationally regulated gene depends on both translational regulatory signals and underlying transcription rates.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.84.18.6335