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Influence of altered transcription on the translational control of human ferritin expression
In this paper, we examine the response of a translational regulatory mechanism when changes in mRNA levels are induced. The gene that encodes the human ferritin heavy chain has been transfected into mouse fibroblasts. Stable transformants that express the human ferritin heavy chain have been isolate...
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| Published in: | Proceedings of the National Academy of Sciences - PNAS 1987-09, Vol.84 (18), p.6335-6339 |
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| container_end_page | 6339 |
| container_issue | 18 |
| container_start_page | 6335 |
| container_title | Proceedings of the National Academy of Sciences - PNAS |
| container_volume | 84 |
| creator | Rouault, T.A Hentze, M.W Dancis, A Caughman, W Harford, J.B Klausner, R.D |
| description | In this paper, we examine the response of a translational regulatory mechanism when changes in mRNA levels are induced. The gene that encodes the human ferritin heavy chain has been transfected into mouse fibroblasts. Stable transformants that express the human ferritin heavy chain have been isolated. This protein assembles into ferritin polymers and can co-assemble with host mouse ferritin. Biosynthetic rates of the expressed human ferritin varied over a wide range in response to perturbations in iron supply, but total and cytoplasmic messenger RNA levels remained unchanged. When changes in ferritin mRNA levels were induced by treatment with sodium butyrate, proportional changes in the biosynthetic rates of ferritin were observed, but the capacity for modulating biosynthesis in response to alterations in iron availability was preserved. These findings suggest that the final protein biosynthetic rate of a translationally regulated gene depends on both translational regulatory signals and underlying transcription rates. |
| doi_str_mv | 10.1073/pnas.84.18.6335 |
| format | article |
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The gene that encodes the human ferritin heavy chain has been transfected into mouse fibroblasts. Stable transformants that express the human ferritin heavy chain have been isolated. This protein assembles into ferritin polymers and can co-assemble with host mouse ferritin. Biosynthetic rates of the expressed human ferritin varied over a wide range in response to perturbations in iron supply, but total and cytoplasmic messenger RNA levels remained unchanged. When changes in ferritin mRNA levels were induced by treatment with sodium butyrate, proportional changes in the biosynthetic rates of ferritin were observed, but the capacity for modulating biosynthesis in response to alterations in iron availability was preserved. These findings suggest that the final protein biosynthetic rate of a translationally regulated gene depends on both translational regulatory signals and underlying transcription rates.</description><identifier>ISSN: 0027-8424</identifier><identifier>EISSN: 1091-6490</identifier><identifier>DOI: 10.1073/pnas.84.18.6335</identifier><identifier>PMID: 3476949</identifier><identifier>CODEN: PNASA6</identifier><language>eng</language><publisher>Washington, DC: National Academy of Sciences of the United States of America</publisher><subject>Biological and medical sciences ; BIOSINTESIS ; BIOSYNTHESE ; BIOSYNTHESIS ; Butyrates ; Butyrates - pharmacology ; Butyric Acid ; Cell lines ; Cellular metabolism ; CONTROL GENETICO ; FER ; Ferritins ; Ferritins - genetics ; Fibroblasts ; Fundamental and applied biological sciences. Psychology ; GENE ; Gene expression ; Gene expression regulation ; Gene Expression Regulation - drug effects ; GENERO HUMANO ; GENES ; GENETIC CONTROL ; GENRE HUMAIN ; HIERRO ; Humans ; IRON ; K562 cells ; LUTTE GENETIQUE ; MANKIND ; Messenger RNA ; METABOLISME DES MINERAUX ; METABOLISMO DE MINERALES ; METALLOPROTEINE ; METALLOPROTEINS ; METALPROTEINAS ; MICE ; MINERAL METABOLISM ; Molecular and cellular biology ; Molecular genetics ; Protein Biosynthesis ; RATON ; RNA ; SOURIS ; Transcription, Genetic ; Transfection</subject><ispartof>Proceedings of the National Academy of Sciences - PNAS, 1987-09, Vol.84 (18), p.6335-6339</ispartof><rights>1987 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c569t-e5477a526b2bd2c551f933f91e2b74c5230f6d797a507e0135bbbaa2b887ec3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://www.pnas.org/content/84/18.cover.gif</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/29831$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/29831$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,27924,27925,53791,53793,58238,58471</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=8359129$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/3476949$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Rouault, T.A</creatorcontrib><creatorcontrib>Hentze, M.W</creatorcontrib><creatorcontrib>Dancis, A</creatorcontrib><creatorcontrib>Caughman, W</creatorcontrib><creatorcontrib>Harford, J.B</creatorcontrib><creatorcontrib>Klausner, R.D</creatorcontrib><title>Influence of altered transcription on the translational control of human ferritin expression</title><title>Proceedings of the National Academy of Sciences - PNAS</title><addtitle>Proc Natl Acad Sci U S A</addtitle><description>In this paper, we examine the response of a translational regulatory mechanism when changes in mRNA levels are induced. The gene that encodes the human ferritin heavy chain has been transfected into mouse fibroblasts. Stable transformants that express the human ferritin heavy chain have been isolated. This protein assembles into ferritin polymers and can co-assemble with host mouse ferritin. Biosynthetic rates of the expressed human ferritin varied over a wide range in response to perturbations in iron supply, but total and cytoplasmic messenger RNA levels remained unchanged. When changes in ferritin mRNA levels were induced by treatment with sodium butyrate, proportional changes in the biosynthetic rates of ferritin were observed, but the capacity for modulating biosynthesis in response to alterations in iron availability was preserved. These findings suggest that the final protein biosynthetic rate of a translationally regulated gene depends on both translational regulatory signals and underlying transcription rates.</description><subject>Biological and medical sciences</subject><subject>BIOSINTESIS</subject><subject>BIOSYNTHESE</subject><subject>BIOSYNTHESIS</subject><subject>Butyrates</subject><subject>Butyrates - pharmacology</subject><subject>Butyric Acid</subject><subject>Cell lines</subject><subject>Cellular metabolism</subject><subject>CONTROL GENETICO</subject><subject>FER</subject><subject>Ferritins</subject><subject>Ferritins - genetics</subject><subject>Fibroblasts</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>GENE</subject><subject>Gene expression</subject><subject>Gene expression regulation</subject><subject>Gene Expression Regulation - drug effects</subject><subject>GENERO HUMANO</subject><subject>GENES</subject><subject>GENETIC CONTROL</subject><subject>GENRE HUMAIN</subject><subject>HIERRO</subject><subject>Humans</subject><subject>IRON</subject><subject>K562 cells</subject><subject>LUTTE GENETIQUE</subject><subject>MANKIND</subject><subject>Messenger RNA</subject><subject>METABOLISME DES MINERAUX</subject><subject>METABOLISMO DE MINERALES</subject><subject>METALLOPROTEINE</subject><subject>METALLOPROTEINS</subject><subject>METALPROTEINAS</subject><subject>MICE</subject><subject>MINERAL METABOLISM</subject><subject>Molecular and cellular biology</subject><subject>Molecular genetics</subject><subject>Protein Biosynthesis</subject><subject>RATON</subject><subject>RNA</subject><subject>SOURIS</subject><subject>Transcription, Genetic</subject><subject>Transfection</subject><issn>0027-8424</issn><issn>1091-6490</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1987</creationdate><recordtype>article</recordtype><recordid>eNqFkU1rFTEYhYMo9ba6FkRlFqKruc3nJFm4kOJHoeCiuhNCJjfpnZKb3CYZqf--GWYYdKMQCLznOW9OOAC8QHCLICfnx6DzVtAtEtuOEPYIbBCUqO2ohI_BBkLMW0ExfQpOc76FEEom4Ak4IZR3ksoN-HkZnB9tMLaJrtG-2GR3TUk6ZJOGYxliaOopezsPvZ5G2jcmhpKin1z78aBD42xKQxlCY--PyeZcsWfgidM-2-fLfQauP3_6fvG1vfr25fLi41VrWCdLaxnlXDPc9bjfYcMYcpIQJ5HFPaeGYQJdt-OyMpBbiAjr-15r3AvBrSFn4MO89Tj2B7sztgbTXh3TcNDpt4p6UH8rYdirm_hLYSkhh9X_bvGneDfaXNRhyMZ6r4ONY1acc8Sl6P4LIioI4YxV8HwGTYo5J-vWMAiqqTY11aYEVUioqbbqeP3nH1Z-6anqbxddZ6O9q1WYIa-YIEwiPGHvF2zav6rrO8qNvlZ8Xyr55p9kBV7OwG0uMa0EloKgKr6aRaej0jepRvlxLTiTmAghyAPzGcz3</recordid><startdate>19870901</startdate><enddate>19870901</enddate><creator>Rouault, T.A</creator><creator>Hentze, M.W</creator><creator>Dancis, A</creator><creator>Caughman, W</creator><creator>Harford, J.B</creator><creator>Klausner, R.D</creator><general>National Academy of Sciences of the United States of America</general><general>National Acad Sciences</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19870901</creationdate><title>Influence of altered transcription on the translational control of human ferritin expression</title><author>Rouault, T.A ; Hentze, M.W ; Dancis, A ; Caughman, W ; Harford, J.B ; Klausner, R.D</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c569t-e5477a526b2bd2c551f933f91e2b74c5230f6d797a507e0135bbbaa2b887ec3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1987</creationdate><topic>Biological and medical sciences</topic><topic>BIOSINTESIS</topic><topic>BIOSYNTHESE</topic><topic>BIOSYNTHESIS</topic><topic>Butyrates</topic><topic>Butyrates - pharmacology</topic><topic>Butyric Acid</topic><topic>Cell lines</topic><topic>Cellular metabolism</topic><topic>CONTROL GENETICO</topic><topic>FER</topic><topic>Ferritins</topic><topic>Ferritins - genetics</topic><topic>Fibroblasts</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>GENE</topic><topic>Gene expression</topic><topic>Gene expression regulation</topic><topic>Gene Expression Regulation - drug effects</topic><topic>GENERO HUMANO</topic><topic>GENES</topic><topic>GENETIC CONTROL</topic><topic>GENRE HUMAIN</topic><topic>HIERRO</topic><topic>Humans</topic><topic>IRON</topic><topic>K562 cells</topic><topic>LUTTE GENETIQUE</topic><topic>MANKIND</topic><topic>Messenger RNA</topic><topic>METABOLISME DES MINERAUX</topic><topic>METABOLISMO DE MINERALES</topic><topic>METALLOPROTEINE</topic><topic>METALLOPROTEINS</topic><topic>METALPROTEINAS</topic><topic>MICE</topic><topic>MINERAL METABOLISM</topic><topic>Molecular and cellular biology</topic><topic>Molecular genetics</topic><topic>Protein Biosynthesis</topic><topic>RATON</topic><topic>RNA</topic><topic>SOURIS</topic><topic>Transcription, Genetic</topic><topic>Transfection</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Rouault, T.A</creatorcontrib><creatorcontrib>Hentze, M.W</creatorcontrib><creatorcontrib>Dancis, A</creatorcontrib><creatorcontrib>Caughman, W</creatorcontrib><creatorcontrib>Harford, J.B</creatorcontrib><creatorcontrib>Klausner, R.D</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Rouault, T.A</au><au>Hentze, M.W</au><au>Dancis, A</au><au>Caughman, W</au><au>Harford, J.B</au><au>Klausner, R.D</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Influence of altered transcription on the translational control of human ferritin expression</atitle><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle><addtitle>Proc Natl Acad Sci U S A</addtitle><date>1987-09-01</date><risdate>1987</risdate><volume>84</volume><issue>18</issue><spage>6335</spage><epage>6339</epage><pages>6335-6339</pages><issn>0027-8424</issn><eissn>1091-6490</eissn><coden>PNASA6</coden><abstract>In this paper, we examine the response of a translational regulatory mechanism when changes in mRNA levels are induced. The gene that encodes the human ferritin heavy chain has been transfected into mouse fibroblasts. Stable transformants that express the human ferritin heavy chain have been isolated. This protein assembles into ferritin polymers and can co-assemble with host mouse ferritin. Biosynthetic rates of the expressed human ferritin varied over a wide range in response to perturbations in iron supply, but total and cytoplasmic messenger RNA levels remained unchanged. When changes in ferritin mRNA levels were induced by treatment with sodium butyrate, proportional changes in the biosynthetic rates of ferritin were observed, but the capacity for modulating biosynthesis in response to alterations in iron availability was preserved. These findings suggest that the final protein biosynthetic rate of a translationally regulated gene depends on both translational regulatory signals and underlying transcription rates.</abstract><cop>Washington, DC</cop><pub>National Academy of Sciences of the United States of America</pub><pmid>3476949</pmid><doi>10.1073/pnas.84.18.6335</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| identifier | ISSN: 0027-8424 |
| ispartof | Proceedings of the National Academy of Sciences - PNAS, 1987-09, Vol.84 (18), p.6335-6339 |
| issn | 0027-8424 1091-6490 |
| language | eng |
| recordid | cdi_pubmed_primary_3476949 |
| source | JSTOR-E-Journals; PubMed Central |
| subjects | Biological and medical sciences BIOSINTESIS BIOSYNTHESE BIOSYNTHESIS Butyrates Butyrates - pharmacology Butyric Acid Cell lines Cellular metabolism CONTROL GENETICO FER Ferritins Ferritins - genetics Fibroblasts Fundamental and applied biological sciences. Psychology GENE Gene expression Gene expression regulation Gene Expression Regulation - drug effects GENERO HUMANO GENES GENETIC CONTROL GENRE HUMAIN HIERRO Humans IRON K562 cells LUTTE GENETIQUE MANKIND Messenger RNA METABOLISME DES MINERAUX METABOLISMO DE MINERALES METALLOPROTEINE METALLOPROTEINS METALPROTEINAS MICE MINERAL METABOLISM Molecular and cellular biology Molecular genetics Protein Biosynthesis RATON RNA SOURIS Transcription, Genetic Transfection |
| title | Influence of altered transcription on the translational control of human ferritin expression |
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