A redox cycle with complex II prioritizes sulfide quinone oxidoreductase-dependent H 2 S oxidation

• Published in: The Journal of biological chemistry 2022-01, Vol.298 (1), p.101435
• Main Authors: Kumar, Roshan, Landry, Aaron P, Guha, Arkajit, Vitvitsky, Victor, Lee, Ho Joon, Seike, Keisuke, Reddy, Pavan, Lyssiotis, Costas A, Banerjee, Ruma
• Format: Article
• Language: English
• Subjects: Animals; Electron Transport Complex IV - antagonists & inhibitors; Electron Transport Complex IV - metabolism; Hydrogen Sulfide - metabolism; Mice; Oxidation-Reduction; Quinone Reductases - genetics; Quinone Reductases - metabolism
• ISSN: 1083-351X
• DOI: 10.1016/j.jbc.2021.101435

The dual roles of H S as an endogenously synthesized respiratory substrate and as a toxin raise questions as to how it is cleared when the electron transport chain is inhibited. Sulfide quinone oxidoreductase (SQOR) catalyzes the first step in the mitochondrial H S oxidation pathway, using CoQ as an electron acceptor, and connects to the electron transport chain at the level of complex III. We have discovered that at high H S concentrations, which are known to inhibit complex IV, a new redox cycle is established between SQOR and complex II, operating in reverse. Under these conditions, the purine nucleotide cycle and the malate aspartate shuttle furnish fumarate, which supports complex II reversal and leads to succinate accumulation. Complex II knockdown in colonocytes decreases the efficiency of H S clearance while targeted knockout of complex II in intestinal epithelial cells significantly decreases the levels of thiosulfate, a biomarker of H S oxidation, to approximately one-third of the values seen in serum and urine samples from control mice. These data establish the physiological relevance of this newly discovered redox circuitry between SQOR and complex II for prioritizing H S oxidation and reveal the quantitatively significant contribution of intestinal epithelial cells to systemic H S metabolism.