Evaluation of Traumatic Spinal Cord Injury in a Rat Model Using 99m Tc-GA-5 as a Potential In Vivo Tracer

Spinal cord injury (SCI) refers to the damage suffered in the spinal cord by any trauma or pathology. The purpose of this work was to determine whether Tc-GA-5, a radiotracer targeting Glial Fibrillary Acidic Protein (GFAP), can reveal in vivo the reactivation of astrocytes in a murine model with SC...

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Bibliographic Details
Published in:Molecules (Basel, Switzerland) Switzerland), 2021-11, Vol.26 (23)
Main Authors: Izquierdo-Sánchez, Vanessa, Zambrano-Rodríguez, Pablo C, Peña-Merino, Nadia, Bolaños-Puchet, Sirio, Reyes-Alva, Horacio J, Martínez-Cruz, Angelina, Muñiz-Hernández, Saé, Guízar-Sahagún, Gabriel, Medina, Luis Alberto
Format: Article
Language:English
Subjects:
Animals
Antibodies, Monoclonal - chemistry
Antibodies, Monoclonal - pharmacology
Disease Models, Animal
Glial Fibrillary Acidic Protein - genetics
Glial Fibrillary Acidic Protein - immunology
Glial Fibrillary Acidic Protein - pharmacology
Humans
Radiochemistry
Radiopharmaceuticals - pharmacology
Rats
Single Photon Emission Computed Tomography Computed Tomography
Spinal Cord - diagnostic imaging
Spinal Cord - pathology
Spinal Cord Injuries - diagnostic imaging
Spinal Cord Injuries - genetics
Spinal Cord Injuries - pathology
Technetium - chemistry
Technetium - pharmacology
Tissue Distribution - radiation effects
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Summary:Spinal cord injury (SCI) refers to the damage suffered in the spinal cord by any trauma or pathology. The purpose of this work was to determine whether Tc-GA-5, a radiotracer targeting Glial Fibrillary Acidic Protein (GFAP), can reveal in vivo the reactivation of astrocytes in a murine model with SCI. A method for the Tc radiolabeling of the mouse anti-GFAP monoclonal antibody GA-5 was implemented. Radiochemical characterization was performed, and radioimmunohistochemistry assays were used to evaluate the integrity of Tc-GA-5. MicroSPECT/CT was used for in vivo imaging to trace SCI in the rats. No alterations in the GA-5's recognition/specificity ability were observed after the radiolabeling. The GA-5's radiolabeling procedure implemented in this work offers a practical method to allow the in vivo following of this monoclonal antibody to evaluate its biodistribution and specificity for GFAP receptors using SPECT/CT molecular imaging.
ISSN:1420-3049