Protective effects of galangin against H 2 O 2 /UVB-induced dermal fibroblast collagen degradation via hsa-microRNA-4535-mediated TGFβ/Smad signaling

• Published in: Aging (Albany, NY.) NY.), 2021-12, Vol.13 (23), p.25342
• Main Authors: Lee, Jian-Jr, Ng, Shang-Chuan, Ni, Yean-Tin, Liu, Jian-Sheng, Chen, Chih-Jung, Padma, Viswanadha Vijaya, Huang, Chih-Yang, Kuo, Wei-Wen
• Format: Article
• Language: English
• Subjects: Collagen - drug effects; Collagen - metabolism; Collagen - radiation effects; Fibroblasts - drug effects; Fibroblasts - metabolism; Fibroblasts - radiation effects; Flavonoids - pharmacology; Humans; Hydrogen Peroxide - adverse effects; MicroRNAs - metabolism; Radiation-Protective Agents - pharmacology; Signal Transduction - drug effects; Signal Transduction - radiation effects; Skin - drug effects; Skin - metabolism; Skin - radiation effects; Skin Aging - drug effects; Skin Aging - radiation effects; Smad Proteins - metabolism; Transforming Growth Factor beta - metabolism; Ultraviolet Rays - adverse effects
• ISSN: 1945-4589

This study aimed to investigate the mechanism underlying the protective effects of galangin against H O /UVB-induced damage using and models of photodamage. Moreover, we identified the involvement of miRNA regulation in this process. The H O /UVB-treated HS68 human dermal fibroblasts and UVB-induced C57BL/6J nude mice were used as and models of photodamage. The results showed that galangin treatment alleviated H O /UVB-induced reduction in cell viability, TGFβ/Smad signaling impairment, and dermal aging. Based on the results of microRNA array analyses and database searches, hsa-miR-4535 was identified as a potential candidate miRNA that targets Smad4. , galangin treatment activated Smad2/3/4 complex and inhibited hsa-miR-4535 expression in H2O2/UVB-exposed cells. , topical application of low (12 mg/kg) and high doses (24 mg/kg) of galangin to the dorsal skin of C57BL/6J nude mice significantly alleviated UVB-induced skin photodamage by promoting TGFβ/Smad collagen synthesis signaling, reducing epidermal hyperplasia, wrinkle formation, and skin senescence, as well as inhibiting hsa-miR-4535 expression. Taken together, our findings indicate a link between hsa-miR-4535 and TGFβ/Smad collagen synthesis signaling and suggest these factors to be involved in the photo-protective mechanism of galangin in dermal fibroblasts against H O /UVB-induced aging. The evidence indicated that galangin with anti-aging properties can be considered as a supplement in skin care products.