Fluorescence-based immunochromatographic test strip for the detection of hyoscyamine

• Published in: Analyst (London) 2022-01, Vol.147 (2), p.293-32
• Main Authors: Xu, Xinxin, Ge, Wenliang, Suryoprabowo, Steven, Guo, Xin, Zhu, Jianping, Liu, Liqiang, Xu, Chuanlai, Kuang, Hua
• Format: Article
• Language: English
• Subjects: Animals; Anticholinergics; Assaying; Chromatography, Affinity; Fluorescence; Gold; Hyoscyamine; Limit of Detection; Metal Nanoparticles; Monoclonal antibodies; Nanoparticles; Pork; Screening; Swine
• ISSN: 0003-2654; 1364-5528
• DOI: 10.1039/d1an01973b

Hyoscyamine (HSM), which acts as an antagonist of the acetylcholine muscarinic receptor and can induce a variety of distinct toxic syndromes in mammals (anti-cholinergic poisoning), is hazardous to human health. Therefore, it is urgent to develop a rapid, sensitive, and cost-effective method to determine HSM. A fluorescent microsphere based immunochromatographic assay was developed for this analyte and gold nanoparticles (AuNPs) were used as a comparison. A monoclonal antibody against HSM was prepared with a 50% inhibition concentration (IC 50 ) of 1.17 ng mL −1 , with no cross-reactivity with five drugs. Under optimized conditions, the cut off limits using the fluorescence-labeled monoclonal antibody strips were 10 ng mL −1 in 0.01 M PBS and 20 ng mL −1 in pork, pig urine, and honey samples, and the assay could be completed within 10 min. In comparison with a AuNP immunochromatographic assay, the developed method offered a higher coupling rate and lower amounts of antibodies. This approach could be used for simple, sensitive and rapid screening, and is suitable for on-site screening applications. A fluorescent microsphere based immunochromatographic assay was developed for hyoscyamine. In comparison with a AuNP immunochromatographic assay, the developed method offered a higher coupling rate and lower amounts of antibodies.