Organoarsenic probes to study proteins by NMR spectroscopy

Arsenical probes enable structural studies of proteins. We report the first organoarsenic probes for nuclear magnetic resonance (NMR) and electron paramagnetic resonance (EPR) spectroscopy to study proteins in solutions. These probes can be attached to irregular loop regions. A lanthanide-binding ta...

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Bibliographic Details
Published in:Chemical communications (Cambridge, England) England), 2022-01, Vol.58 (5), p.71-74
Main Authors: Mahawaththa, Mithun C, Orton, Henry W, Adekoya, Ibidolapo, Huber, Thomas, Otting, Gottfried, Nitsche, Christoph
Format: Article
Language:English
Subjects:
Arsenicals - chemistry
Electron paramagnetic resonance
Electron Spin Resonance Spectroscopy
Lanthanoid Series Elements - chemistry
Molecular Probes - chemistry
NMR
NMR spectroscopy
Nuclear magnetic resonance
Nuclear Magnetic Resonance, Biomolecular
Proteins
Proteins - chemistry
Spectroscopic analysis
Spectrum analysis
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Summary:Arsenical probes enable structural studies of proteins. We report the first organoarsenic probes for nuclear magnetic resonance (NMR) and electron paramagnetic resonance (EPR) spectroscopy to study proteins in solutions. These probes can be attached to irregular loop regions. A lanthanide-binding tag induces sizable pseudocontact shifts in protein NMR spectra of a magnitude never observed for small paramagnetic probes before. The first organoarsenic probes for nuclear magnetic resonance spectroscopy are reported, allowing the study of proteins in solutions with high sensitivity.
ISSN:1359-7345
1364-548X
1364-548X
DOI:10.1039/d1cc06497e