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Isolation and Characterization of A2-EPTX-Nsm1a, a Secretory Phospholipase A 2 from Malaysian Spitting Cobra ( Naja sumatrana ) Venom
Phospholipase A (PLA ) toxins are one of the main toxin families found in snake venom. PLA toxins are associated with various detrimental effects, including neurotoxicity, myotoxicity, hemostatic disturbances, nephrotoxicity, edema, and inflammation. Although venom contains substantial quantities of...
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Published in: | Toxins 2021-12, Vol.13 (12) |
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Main Authors: | , , , , , |
Format: | Article |
Language: | English |
Subjects: | |
Online Access: | Get full text |
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Summary: | Phospholipase A
(PLA
) toxins are one of the main toxin families found in snake venom. PLA
toxins are associated with various detrimental effects, including neurotoxicity, myotoxicity, hemostatic disturbances, nephrotoxicity, edema, and inflammation. Although
venom contains substantial quantities of PLA
components
there is limited information on the function and activities of PLA
toxins from the venom. In this study, a secretory PLA
from the venom of Malaysian
, subsequently named A2-EPTX-Nsm1a, was isolated, purified, and characterized. A2-EPTX-Nsm1a was purified using a mass spectrometry-guided approach and multiple chromatography steps. Based on LC-MSMS, A2-EPTX-Nsm1a was found to show high sequence similarity with PLA
from venoms of other
species. The PLA
activity of A2-EPTX-Nsm1 was inhibited by 4-BPB and EDTA. A2-EPTX-Nsm1a was significantly less cytotoxic in a neuroblastoma cell line (SH-SY5Y) compared to crude venom and did not show a concentration-dependent cytotoxic activity. To our knowledge, this is the first study that characterizes and investigates the cytotoxicity of an Asp49 PLA
isolated from Malaysian
venom in a human neuroblastoma cell line. |
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ISSN: | 2072-6651 |