Ethanol promoting the upregulation of C-X-C Motif Chemokine Ligand 1（CXCL1） and C-X-C Motif Chemokine Ligand 6（CXCL6） in models of early alcoholic liver disease

• Published in: Bioengineered 2022-03, Vol.13 (3), p.4688-4701
• Main Authors: Jiang, Yao, Xi, Yuge, Li, Yiqin, Zuo, Zhihua, Zeng, Chuyi, Fan, Jia, Zhang, Dan, Tao, Hualin, Guo, Yongcan
• Format: Article
• Language: English
• Subjects: Alcoholic hepatitis· alcoholic liver disease· CXCL1· CXCL6· immune-related genes; Animals; Ethanol; Ligands; Liver - metabolism; Liver Diseases, Alcoholic - genetics; Liver Diseases, Alcoholic - metabolism; Mice; Mice, Inbred C57BL; Research Paper; RNA, Messenger - genetics; Up-Regulation - genetics
• ISSN: 2165-5979; 2165-5987
• DOI: 10.1080/21655979.2022.2030557

Alcoholic liver disease (ALD) denotes a series of liver diseases caused by ethanol. Recently, immune-related genes (IRGs) play increasingly crucial role in diseases. However, it's unclear the role of IRGs in ALD. Bioinformatic analysis was used to discern the core immune-related differential genes (IRDGs) in the present study. Subsequently, Cell Counting Kit-8 say, oil red O staining, and triglyceride detection were employed to explore optimal experimental conditions of establishing hepatocellular models of early ALD. Ultimately, real-time reverse transcription-PCR and immunohistochemistry/immunocytochemistry methods were adopted to verify the expressions of mRNA and proteins of core IRDGs, respectively. C-X-C Motif Chemokine Ligand 1 (Cxcl1) and Cxcl6 were regarded as core IRDGs via integrated bioinformatics analysis. Besides, Lieber Decarli Ethanol feeding and 200 mM and 300 mM ethanol stimulating L02 cells for 36 h can both successfully hepatocellular model. In ethanol groups, the levels of CXCL1 and CXCL6 mRNA were significantly upregulated than pair-fed groups (P