Effect of hydrated C 60 fullerene on lipid, vitamin and amino acid composition in frozen-thawed ram semen

• Published in: Animal reproduction science 2022-03, Vol.238, p.106939
• Main Authors: Türk, Gaffari, Koca, Recep H, Güngör, İbrahim H, Dayan Cinkara, Serap, Acısu, Tutku C, Erdem Erişir, Figen, Arkalı, Gözde, Özer Kaya, Şeyma, Kızıl, Meltem, Sönmez, Mustafa, Gür, Seyfettin, Yılmaz, Ökkeş, Yüce, Abdurrauf, Karatepe, Mustafa
• Format: Article
• Language: English
• Subjects: Amino Acids - pharmacology; Animals; Cryopreservation - veterinary; Cryoprotective Agents - chemistry; Cryoprotective Agents - pharmacology; Fullerenes - pharmacology; Lipids; Seeds; Semen; Semen Preservation - veterinary; Sheep; Sperm Motility; Spermatozoa; Vitamins - pharmacology
• ISSN: 1873-2232

This study was conducted to investigate the effect of different doses of hydrated C fullerene (C HyFn) on freeze-thawing process-induced changes in lipid, vitamin and amino acid composition and also in motility, kinematic, sperm quality and oxidative stress parameters in ram semen. Semen was collected from seven rams twice a week for 3 weeks, so six repetitions were performed. The semen collected in each repetition was pooled. Each pooled sample was diluted with tris + egg yolk extender with (200 nM, 400 nM, 800 nM, 1 µM and 5 µM) and without (control) C HyFn and they were frozen in mini straws. The doses of 800 nM, 1 µM and 5 µM had higher total, progressive motility, sperm membrane functionality rates, glutathione-peroxidase and catalase activities. All doses of C HyFn significantly reduced dead and total abnormal sperm rates and malondialdehyde levels. Significant increases in vitamin A (400 and 800 nM doses), vitamin K (400 nM, 800 nM and 1 µM doses), total amino acid (all doses) levels, but significant decreases in vitamin D (800 nM, 1 and 5 µM doses), vitamin D (1 and 5 µM doses) and vitamin E (200 nM, 1 and 5 µM) levels were observed compared to control. In conclusion, the addition of C HyFn to ram semen at 200 nM - 5 µM range, especially at a dose of 800 nM, provides a positive contribution to the protection of motility, vitamins A, K and total amino acid levels, and oxidant/antioxidant balance after freeze-thawing.