Changes in colostrum ingredients of Hu sheep, as well as the missense mutation genes associated with colostrum yield

• Published in: Animal biotechnology 2023-11, Vol.34 (4), p.1492-1504
• Main Authors: Zhou, Aimin, Chong, Yuqing, Liu, Guiqiong, Jiang, Xunping, Huang, Yongjie, Bo, Dongdong, Guo, Qiusong, Hu, Ruixue, Chi, Shaxuan, Wang, Mingjing, Yan, Yinan, Sun, Ling, Mao, Xin
• Format: Article
• Language: English
• Subjects: Animal health; Animal husbandry; candidate gene; Colostrum; colostrum composition; Evolutionary genetics; Genes; Genomes; Hu sheep; immunity; Immunoglobulin A; Immunoglobulin G; Immunoglobulin M; Lactose; Litter size; Missense mutation; Nutrients; Postpartum; Proteins; Sheep; Signal detection; Src protein
• ISSN: 1049-5398; 1532-2378
• DOI: 10.1080/10495398.2022.2034641

This study focused on the changes in the composition and immune evolution in milk from birth to 144 h postpartum and the genes associated with the colostrum yield of Hu sheep. Twelve Hu sheep, which were bred carefully under animal health standards and have a litter size of two kids and similar gestation length (149 ± 1 days), were used. Lambs were transferred into their own cots to avoid interference. The compositional content (i.e., fat, protein, and lactose) and some other properties, including daily colostrum yield, DM, and SNF, were determined. In addition, immunity molecules (IgG, IgA, and IgM concentrations) received remarkable attention. The DM, SNF, fat, and protein contents were higher in the first days postpartum and then dropped quickly from the time of birth to 144 h postpartum. However, the lactose content displayed an increasing pattern and reached normal milk percentage at 48 h. The highest IgG (103.17 mg/mL), IgA (352.82 μg/mL), and IgM (2.79 mg/mL) colostrum concentrations were observed at partum, decreased quickly, and finally stabilized. The change law of concentration of IgA and IgM in colostrum are the same with IgG. Furthermore, the whole-genome resequencing was performed, and a missense variant locus in the SRC gene and two missense locus variants in the HIF1A gene were significantly associated with the colostrum yield of sheep by using the whole-genome selection signal detection analysis. In conclusions, colostrum contains abundant nutrients especially immunoglobulin, and the HIF1A gene may be used as candidate genes for colostrum yield, which has important information as a basic knowledge for the Hu sheep breeding program.