Reversible single cell trapping of Paramecium caudatum to correlate swimming behavior and membrane state

Single cell measurements with living specimen like, for example, the ciliated protozoan Paramecium caudatum can be a challenging task. We present here a microfluidic trapping mechanism for measurements with these micro-organisms that can be used, e.g., for optical measurements to correlate cellular...

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Bibliographic Details
Published in:Biomicrofluidics 2022-03, Vol.16 (2), p.024102-024102
Main Authors: Schnitzler, Lukas G., Paeger, Anne, Brugger, Manuel S., Schneider, Matthias F., Westerhausen, Christoph
Format: Article
Language:English
Subjects:
Fluorescent dyes
Lipids
Membranes
Microfluidics
Optical measurement
Protozoa
Regular
Swimming
Trapping
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Summary:Single cell measurements with living specimen like, for example, the ciliated protozoan Paramecium caudatum can be a challenging task. We present here a microfluidic trapping mechanism for measurements with these micro-organisms that can be used, e.g., for optical measurements to correlate cellular functions with the phase state of the lipid membrane. Here, we reversibly trap single cells in small compartments. Furthermore, we track and analyze the swimming behavior of single cells over several minutes. Before and after reversible trapping the swimming speed is comparable, suggesting that trapping does not have a large effect on cell behavior. Last, we demonstrate the feasibility of membrane order measurements on living cells using the fluorescent dye 6-lauryl-2-dimethylaminonaphthalene (Laurdan).
ISSN:1932-1058
1932-1058
DOI:10.1063/5.0084084