Immunoassay-aptasensor for the determination of tumor-derived exosomes based on the combination of magnetic nanoparticles and hybridization chain reaction

The detection of tumor-related exosomes is of great significance. In this work, a fluorescence aptasensor was designed for the determination of tumor-related exosomes based on the capture of magnetic nanoparticles (MNPs) and specific recognition of an aptamer. MNPs were used as substrates to capture...

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Bibliographic Details
Published in:RSC advances 2021-01, Vol.11 (9), p.4983-499
Main Authors: Zhang, Hua, Zhou, Yajuan, Luo, Dan, Liu, Jingjian, Yang, E, Yang, Guangyi, Feng, Guangjun, Chen, Qinhua, Wu, Lun
Format: Article
Language:English
Subjects:
Antibodies
Biological properties
Chemistry
Fluorescence
Immunoassay
Linearity
Nanoparticles
Substrates
Tumors
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Summary:The detection of tumor-related exosomes is of great significance. In this work, a fluorescence aptasensor was designed for the determination of tumor-related exosomes based on the capture of magnetic nanoparticles (MNPs) and specific recognition of an aptamer. MNPs were used as substrates to capture the exosomes by modifying the CD63 antibody on the MNP surface. Probe 1 consists of PDL-1 aptamer sequence and a section of other sequences. PDL-1 expression was observed on the surface of exosomes; the aptamer of PDL-1 could combine with PDL-1 with high affinity. Thus, the immunoassay-type compounds of "MNPs-exosomes-probe 1" were formed. The other section of probe 1 triggered the HCR with probe 2 and probe 3 and formed the super-long dsDNA. The addition of GelRed resulted in the generation of an amplified fluorescence signal. The proposed design demonstrated a good linearity with the exosome concentration ranging from 300 to 10 7 particles per mL and with a low detection limit of 100 particles per mL. This aptasensor also exhibited high specificity for tumor-related exosomes, and was successfully applied in biological samples. The detection of tumor-related exosomes is of great significance.
ISSN:2046-2069
2046-2069
DOI:10.1039/d0ra10159a