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Compartmentalized cross-linked enzyme nano aggregates (-CLEAs) toward pharmaceutical transformations
A new immobilization strategy using compartmentalized nanoreactors is herein reported for two biocatalytic processes: (1) N -acetylneuraminate lyase (NAL) is internalized in NAL- c -CLE n As and used in a continuous flow aldol condensation of N -acetyl- d -mannosamine with sodium pyruvate to N -acet...
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Published in: | RSC advances 2021-06, Vol.11 (35), p.21857-21861 |
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Main Authors: | , , , , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that this one cites Items that cite this one |
Online Access: | Get full text |
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Summary: | A new immobilization strategy using compartmentalized nanoreactors is herein reported for two biocatalytic processes: (1)
N
-acetylneuraminate lyase (NAL) is internalized in NAL-
c
-CLE
n
As and used in a continuous flow aldol condensation of
N
-acetyl-
d
-mannosamine with sodium pyruvate to
N
-acetylneuraminic acid; (2) two hydroxysteroid dehydrogenases (HSDH) 7α- and 7β-HSDH are incorporated in
c
-CLE
n
As and used in a two-step cascade batch synthesis of ursodeoxycholic acid (UDCA). The versatile use of
c
-CLE
n
A demonstrates that this immobilization methodology is a valuable addition to the toolbox of synthetic chemists.
A new immobilization strategy using compartmentalized nanoreactors is herein reported for two biocatalytic processes: (1) a continuous flow aldol reaction; (2) epimerization for the synthesis of ursodeoxycholic acid (UDCA). |
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ISSN: | 2046-2069 2046-2069 |
DOI: | 10.1039/d1ra04332c |