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Compartmentalized cross-linked enzyme nano aggregates (-CLEAs) toward pharmaceutical transformations

A new immobilization strategy using compartmentalized nanoreactors is herein reported for two biocatalytic processes: (1) N -acetylneuraminate lyase (NAL) is internalized in NAL- c -CLE n As and used in a continuous flow aldol condensation of N -acetyl- d -mannosamine with sodium pyruvate to N -acet...

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Bibliographic Details
Published in:RSC advances 2021-06, Vol.11 (35), p.21857-21861
Main Authors: de Martino, M. Teresa, Tonin, Fabio, Bloemendal, Victor R. L. J, Hanefeld, Ulf, Rutjes, Floris P. J. T, van Hest, Jan C. M
Format: Article
Language:English
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Summary:A new immobilization strategy using compartmentalized nanoreactors is herein reported for two biocatalytic processes: (1) N -acetylneuraminate lyase (NAL) is internalized in NAL- c -CLE n As and used in a continuous flow aldol condensation of N -acetyl- d -mannosamine with sodium pyruvate to N -acetylneuraminic acid; (2) two hydroxysteroid dehydrogenases (HSDH) 7α- and 7β-HSDH are incorporated in c -CLE n As and used in a two-step cascade batch synthesis of ursodeoxycholic acid (UDCA). The versatile use of c -CLE n A demonstrates that this immobilization methodology is a valuable addition to the toolbox of synthetic chemists. A new immobilization strategy using compartmentalized nanoreactors is herein reported for two biocatalytic processes: (1) a continuous flow aldol reaction; (2) epimerization for the synthesis of ursodeoxycholic acid (UDCA).
ISSN:2046-2069
2046-2069
DOI:10.1039/d1ra04332c