Perfluorooctanoic acid - induced lipid metabolism disorder in SD rat liver and its effect on the expression of fatty acid metabolism - related proteins

• Published in: Zhong nan da xue xue bao. Journal of Central South University. Yi xue ban 2022-01, Vol.47 (1), p.18
• Main Authors: Wang, Li, Zhou, Yongbing, Ma, Xinzhuang, Sun, Weiqiang, Liu, Hui
• Format: Article
• Language: chi ; eng
• Subjects: Animals; Caprylates; Fatty Acids - metabolism; Fatty Acids - pharmacology; Fluorocarbons; Lipid Metabolism; Lipid Metabolism Disorders - chemically induced; Lipid Metabolism Disorders - metabolism; Liver - metabolism; Male; PPAR gamma; Rats; Rats, Sprague-Dawley; Sirtuin 1 - metabolism
• ISSN: 1672-7347
• DOI: 10.11817/j.issn.1672-7347.2022.210320

Perfluorooctanoic acid (PFOA) can cause lipid metabolism disorders in animal body and affect the lipolysis and synthesis of fatty acids. Peroxisome proliferators-activated receptor (PPAR) plays an extremely important role in this process. This study aims to explore the effects of PFOA on liver lipid metabolism disorders in Sprague Dewley (SD) rats and the expression of PPAR. A total of 40 male SD rats were randomly divided into 4 groups ( =10 in each group): a control group (ddH O), a low-dose PFOA group [PFOA 1.25 mg/(kg·d)], a middle-dose PFOA group [PFOA 5.00 mg/(kg·d)], and a high-dose PFOA group [PFOA 20.00 mg/(kg·d)]. The rats were fed with normal diet, and PFOA exposure were performed by oral gavage for 14 days, and the rats were observed, weighted and recorded every day during the exposure. After the exposure, the blood was collected, and the livers were quickly stripped after the rats were killed. Part of the liver tissues were fixed in 4% paraformaldehyde for periodic acid-schiff (PAS) staining; the contents of HDLC, LDLC, TG, TC in serum and liver tissues, as well as the activities of their related enzymes were assayed; The expression levels of cyclic adenosine monophosphate-response element binding protein (Cbp), general control of amino acid synthesis 5-like 2 (Gcn5L2), peroxidation peroxisome proliferation factor activated receptor γ (PPAR), silent information regulator 1 (Sirt1) and human retinoid X receptor alpha 2 (Rxrα2) ) were detected by Western blotting. After 14 days of PFOA exposure, the PAS staining positive particles in the cytoplasm and nucleus of SD rats in the medium and high dose groups were significantly reduced compared with the control group. The serum levels of LDLC and TC in the low-dose and middle-dose groups were significantly reduced compared with the control group (all 0.05), there was no significant difference in HDLC and TG (both >0.05). The activities of alkaline phosphatase (AKP) and alanine aminotransferase (ALT) were increased significantly (both