Early line-probe assay using DNA specimens in patients with pulmonary TB

BACKGROUND: We investigated the feasibility of early line-probe assay (LPA) using remnant DNA of Mycobacterium tuberculosis from polymerase chain reaction (PCR) test.METHODS: M. tuberculosis DNA specimens with cycle threshold (Ct) values reported and collected from patients with known results for bo...

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Bibliographic Details
Published in:The international journal of tuberculosis and lung disease 2022-06, Vol.26 (6), p.509-515
Main Authors: Shin, A. Y., Jekarl, D. W., Kim, H. W., Ha, J. H., Ahn, J. H., Kim, J. S.
Format: Article
Language:English
Subjects:
Deoxyribonucleic acid
DNA
Feasibility
Genotype
Isoniazid
Microbial Sensitivity Tests
Multidrug-Resistant
Phenotypes
Polymerase chain reaction
Rifampin
Sensitivity And Specificity
Subgroups
Tuberculosis
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Summary:BACKGROUND: We investigated the feasibility of early line-probe assay (LPA) using remnant DNA of Mycobacterium tuberculosis from polymerase chain reaction (PCR) test.METHODS: M. tuberculosis DNA specimens with cycle threshold (Ct) values reported and collected from patients with known results for both LPA with culture isolates and phenotype drug susceptibility testing (pDST) were selected. The diagnostic performance of DNA-based LPA according to the Ct value was investigated.RESULTS: A total of 143 respiratory specimens were included. For isoniazid resistance, the accuracy in subgroups with Ct value
ISSN:1027-3719
1815-7920
DOI:10.5588/ijtld.21.0381