Genome wide analysis identifies Single Nucleotide Polymorphism variations and altered pathways associated with poor semen quality in breeding bulls

The reason for poor semen quality among the breeding bulls is not well understood. In the present study, we performed high throughput RNAseq analysis of spermatozoa to identify the SNPs present in good and poor quality semen producing Holstein Friesian breeding bulls. A total of 21360 and 44650 SNPs...

Full description

Saved in:
Bibliographic Details
Published in:Reproduction in domestic animals 2022-06
Main Authors: King, John Peter Ebenezer Samuel, Kumaresan, Arumugam, Talluri, Thirumala Rao, Sinha, Manish Kumar, Raval, Kathan, Nag, Pradeep, Karuthadurai, Thirumalaisamy, Aranganathan, V
Format: Article
Language:English
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The reason for poor semen quality among the breeding bulls is not well understood. In the present study, we performed high throughput RNAseq analysis of spermatozoa to identify the SNPs present in good and poor quality semen producing Holstein Friesian breeding bulls. A total of 21360 and 44650 SNPs were identified in good and poor quality semen with a minimum read depth of 20, among which 4780 and 8710 novel variants were observed in good and poor quality semen, respectively. Greater SNPs and indels variations were observed in poor compared to good quality semen. In poor quality semen, SNP variations were observed in ZNF280B, SLC26A2, DMXL1, OR52A1, MACROD2 and REV1 genes, which are associated with regulation of spermatogenesis, post-testicular maturation, Cl channel activity, V-ATPase-mediated intracellular vesicle acidification, a mono-ADP-ribosyl hydrolase and ATR-Chk1 checkpoint activation. GO analysis of filtered genes with significant variations between good and poor quality semen showed enrichment in important pathways related to semen quality such as MAPK signalling pathway, Akt signalling pathway, focal adhesion, cAMP signalling pathway, and Rap1 signalling pathway. Network analysis of filtered genes in poor quality semen showed variations in pathways of purine metabolism, pyrimidine metabolism, prolactin signalling pathway and RNA cap binding complex. It is inferred that SNP in genes involved in maintaining sperm functions could be the reason for poor quality semen production in bulls, and the identified SNPs holds potential to be used as biomarkers for semen quality in bulls.
ISSN:1439-0531