DNA Sequence from the Histidine Operon Control Region: Seven Histidine Codons in a Row

The DNA sequence of 250 base pairs preceding the first structural gene of the histidine operon of Salmonella typhimurium was determined by the dideoxy chain-termination method. Single-stranded DNA template was provided by an M13-histidine transducing phage constructed for the purpose by in vitro rec...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1978-09, Vol.75 (9), p.4281-4285
Main Author: Barnes, Wayne M.
Format: Article
Language:English
Subjects:
Attenuators
Base Sequence
Binding Sites
Codon
Codons
DNA
DNA, Bacterial - genetics
Genes, Regulator
Histidine - genetics
Nucleotide sequences
Operator regions
Operon
Operons
Ribosomes
Ribosomes - metabolism
RNA
Salmonella typhimurium - genetics
Terminator regions
Transcriptional regulatory elements
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Summary:The DNA sequence of 250 base pairs preceding the first structural gene of the histidine operon of Salmonella typhimurium was determined by the dideoxy chain-termination method. Single-stranded DNA template was provided by an M13-histidine transducing phage constructed for the purpose by in vitro recombination. The termination site for the histidine leader RNA is identified by analogy with the trp operon leader termination sequence, and is 47 nucleotides before the start codon of the first structural gene G. Beginning 150 nucleotides before the end of the presumed leader RNA is a possible short protein-coding region with seven histidine codons in a row. It is proposed that the major mechanism of histodine operon control must involve a ribosome arrested at this run of histidine codons when histidine is limiting.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.75.9.4281