Heterogeneity of T cells and macrophages in chlorine-induced acute lung injury in mice using single-cell RNA sequencing

Chlorine (Cl 2 ), as an asphyxiant toxicant, induced poisoning incidents and acute lung injury (ALI) occur frequently. The specific pathogenesis of Cl 2 -induced ALI remains unclear. Immune cells play an important role in the process of lung damage. We used single-cell RNA sequencing (scRNA-seq) tec...

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Bibliographic Details
Published in:Inhalation toxicology 2022-12, Vol.34 (13-14), p.399-411
Main Authors: Zhao, Chen-qian, Liu, Jiang-zheng, Liu, Meng-meng, Ren, Xiao-ting, Kong, De-qin, Peng, Jie, Cao, Meng, Liu, Rui, Hai, Chun-xu, Zhang, Xiao-di
Format: Article
Language:English
Subjects:
acute lung injury
Acute Lung Injury - chemically induced
Acute Lung Injury - genetics
Animals
Anti-Inflammatory Agents - pharmacology
Chlorine
Chlorine - toxicity
Female
Lipopolysaccharides - toxicity
Lung - pathology
Macrophages
Mice
Mice, Inbred BALB C
scRNA-seq
Sequence Analysis, RNA
t cells
T-Lymphocytes
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Summary:Chlorine (Cl 2 ), as an asphyxiant toxicant, induced poisoning incidents and acute lung injury (ALI) occur frequently. The specific pathogenesis of Cl 2 -induced ALI remains unclear. Immune cells play an important role in the process of lung damage. We used single-cell RNA sequencing (scRNA-seq) technology to explore T cells and macrophages molecular mechanism. Female BALB/c mice were exposed to 400 ppm Cl 2 for 15 min. scRNA-seq technology was used to observe the heterogeneity of T cells and macrophages. Hematoxylin-eosin (H&E) staining was used to evaluate the degree of lung injury. Immunofluorescence was used to verify the highly expressed genes of our interest. A total of 5316 to 7742 cells were classified into eight different cell types. Several new highly expressed anti-inflammatory and pro-inflammatory genes were found in T cells and macrophages, which were further verified in vitro. Through the pseudotime analysis of macrophages, it was found that the expression of pro-inflammatory and anti-inflammatory genes showed opposite trends in the development of Cl 2 -induced ALI. This study also mapped T cells-macrophage communication and identified the development of several important receptor-ligand complexes in Cl 2 -induced ALI. These findings are worthy of further exploration and provide new resources and directions for the study of Cl 2 -induced ALI in mice, especially in immune and inflammation mechanisms.
ISSN:0895-8378
1091-7691
DOI:10.1080/08958378.2022.2134526