Royal jelly arranges apoptotic and oxidative stress pathways and reduces damage to liver tissues of rats by down-regulation of Bcl-2, GSK3 and NF-κB and up-regulation of caspase and Nrf-2 protein signalling pathways

Introduction Royal jelly (RJ) from the honey bee, Apis mellifera, is a traditional product that is widely used as a food supplement to support the medical treatment of various diseases. Material and methods Our study continued for 8 weeks. 42 Wistar albino (8 weeks old) male rats were used in the st...

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Published in:Biomarkers 2023-03, Vol.28 (2), p.217-226
Main Authors: Aslan, Abdullah, Gok, Ozlem, Beyaz, Seda, Parlak, Gozde, Can, Muhammed Ismail, Gundogdu, Ramazan, Baspinar, Serpil, Ozercan, Ibrahim Hanifi, Parlak, Akif Evren
Format: Article
Language:English
Subjects:
Animals
Antioxidants - metabolism
Antioxidants - pharmacology
Caspases
Down-Regulation
Fluoride
Glutathione - metabolism
Glycogen Synthase Kinase 3 - metabolism
Liver - metabolism
liver damage
Male
NF-kappa B - metabolism
NF-κB
Nrf-2
Oxidative Stress
Rats
Rats, Wistar
royal jelly
Up-Regulation
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Summary:Introduction Royal jelly (RJ) from the honey bee, Apis mellifera, is a traditional product that is widely used as a food supplement to support the medical treatment of various diseases. Material and methods Our study continued for 8 weeks. 42 Wistar albino (8 weeks old) male rats were used in the study. The study included 6 groups; Group 1: Control group (fed with standard diet), Group 2: RJ (100 mg/kg, bw), Group 3: F-50 (50 mg/kg, bw), group 4: F-100 (100 mg/kg, bw) group 5: F-50 (50 mg/kg, bw) + RJ (100 mg/kg, bw) Group 6: F-100 (100 mg/kg, bw) + RJ (100 mg/kg, bw). Malondialdehyde (MDA), catalase (CAT) and glutathione (GSH) activities in liver tissue were determined by spectrophotometer. Liver tissue samples were examined histopathologically and various protein levels were determined by Western blotting technique. Results RJ caused a significant decrease in MDA level, Bcl-2, GSK3 and NF-κB protein expression levels, whereas induced a significant increase in GSH level, CAT activities and Bax, BDNF, caspase-6, caspase-3, Nrf-2 protein expression levels. Conclusion Our findings suggest RJ to be used as a hepatoprotective agent in the clinic to modulate the toxic effects of fluoride and other chemicals in the future.
ISSN:1354-750X
1366-5804
DOI:10.1080/1354750X.2022.2159526