Augmentation by resveratrol of the inhibitory effect of ethanol on platelet aggregation

• Published in: Platelets (Edinburgh) 2023-12, Vol.34 (1), p.2151995-2151995
• Main Authors: Ekawa, Kazumi, Marumo, Mikio, Wakabayashi, Ichiro
• Format: Article
• Language: English
• Subjects: Calcium; ethanol; platelet aggregation; resveratrol; thromboxane A; thromboxane a2
• ISSN: 0953-7104; 1369-1635
• DOI: 10.1080/09537104.2022.2151995

Ethanol and resveratrol have been shown to inhibit platelet aggregation. The aim of this study was to determine whether resveratrol has an additional effect on ethanol-induced inhibition of platelet aggregation. Ca 2+ entry and subsequent aggregation of human platelets were measured by the fluorescence method and light transmittance method, respectively. Thromboxane B 2 concentrations in media containing platelets were measured by using the enzyme-linked immunosorbent assay. Platelet aggregation induced by thrombin (0.025 U/ml) was significantly inhibited by preincubation of platelets with ethanol (0.5%). Preincubation with resveratrol (3.125 µM), which did not affect thrombin-induced platelet aggregation, significantly augmented the inhibitory effect of ethanol on platelet aggregation. Similar synergic effects of ethanol and resveratrol were found on aggregatory responses to collagen (2 µg/ml) and arachidonic acid (0.25 mM). On the other hand, the thrombin-induced increase in intracellular Ca 2+ concentration ([Ca 2+ ] i ) was not affected by ethanol alone, resveratrol alone or both ethanol and resveratrol together. In nominally Ca 2+ -free medium, arachidonic acid (0.75 mM) caused a potent platelet aggregation, which was not affected by the presence of ethanol alone, resveratrol alone, or both of them together. Thromboxane B 2 formation induced by thrombin was significantly inhibited by ethanol (0.5%) alone and resveratrol (3.125 µM) alone, and these inhibitory effects were significantly augmented in the presence of both ethanol and resveratrol together. Resveratrol shows an additive effect on ethanol-induced inhibition of platelet aggregation. This effect by resveratrol is partly explained by its inhibitory action on thromboxane A 2 production in platelets. In addition, both ethanol and resveratrol attenuate platelet aggregation through acting on the Ca 2+ -dependent intra-platelet pathway after an increase in [Ca 2+ ] i induced by thrombin.