The profiles of durian (Durio zibethinus Murr.) shell phenolics and their antioxidant effects on H 2 O 2 -treated HepG2 cells as well as the metabolites and organ distribution in rats

Durian is a nutritious tropical fruit with potent antioxidant, anti-inflammatory, antibacterial and anti-cancer effects. However, the durian shell was mainly discarded as waste, while there were few studies on the characterization of its phenolic profiles, antioxidant activities, and in vivo metabol...

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Bibliographic Details
Published in:Food research international 2023-01, Vol.163, p.112122
Main Authors: He, Yangzheng, Peng, Li, Xiong, Hua, Liu, Wenqiang, Zhang, Hua, Peng, Xin, Zhu, Xuemei, Guo, Fanghua, Sun, Yong
Format: Article
Language:English
Subjects:
Animals
Antioxidants - analysis
Bombacaceae - chemistry
Hep G2 Cells
Humans
Hydrogen Peroxide - metabolism
Phenols - chemistry
Proto-Oncogene Proteins c-bcl-2 - metabolism
Rats
Tandem Mass Spectrometry
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Summary:Durian is a nutritious tropical fruit with potent antioxidant, anti-inflammatory, antibacterial and anti-cancer effects. However, the durian shell was mainly discarded as waste, while there were few studies on the characterization of its phenolic profiles, antioxidant activities, and in vivo metabolites. In the present study, a total of 17 compounds were identified in durian shell extract (DSE) by using an ultra-high-performance liquid chromatography coupled with linear ion trap quadrupole Orbitrap mass spectrometry (UHPLC-LTQ-Orbitrap-MS/MS), while 33 metabolites were found in rats' plasma, urine and organ. Moreover, DSE could effectively reduce H O -induced oxidative damage in HepG2 cells, reduce the expression of Reactive Oxygen Species (ROS), Malondialdehyde (MDA) and Lactate Dehydrogenase (LDH) and inhibit apoptosis by regulating the expression of Bcl-2-Associated X (BAX), B-Cell Lymphoma 2 (BCL-2), Caspase-3 and Caspase-9 genes and proteins related to mitochondrial pathway apoptosis. This is the first comprehensive report on Durian shell phenolics, their metabolic profiles and underlying mechanisms of the in vitro antioxidant activities.
ISSN:1873-7145