Advances in the metabolic engineering of Saccharomyces cerevisiae and Yarrowia lipolytica for the production of β-carotene

β-Carotene is one kind of the most important carotenoids. The major functions of β-carotene include the antioxidant and anti-cardiovascular properties, which make it a growing market. Recently, the use of metabolic engineering to construct microbial cell factories to synthesize β-carotene has become...

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Bibliographic Details
Published in:Critical reviews in biotechnology 2024-04, Vol.ahead-of-print (ahead-of-print), p.1-15
Main Authors: Guo, Qi, Peng, Qian-Qian, Li, Ya-Wen, Yan, Fang, Wang, Yue-Tong, Ye, Chao, Shi, Tian-Qiong
Format: Article
Language:English
Subjects:
antioxidants
biosynthesis
biotechnology
carotenoids
fermentation
genetic engineering
markets
metabolic engineering
microbial cell factories
Saccharomyces cerevisiae
Yarrowia lipolytica
β-Carotene
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Summary:β-Carotene is one kind of the most important carotenoids. The major functions of β-carotene include the antioxidant and anti-cardiovascular properties, which make it a growing market. Recently, the use of metabolic engineering to construct microbial cell factories to synthesize β-carotene has become the latest model for its industrial production. Among these cell factories, yeasts including Saccharomyces cerevisiae and Yarrowia lipolytica have attracted the most attention because of the: security, mature genetic manipulation tools, high flux toward carotenoids using the native mevalonate pathway and robustness for large-scale fermentation. In this review, the latest strategies for β-carotene biosynthesis, including protein engineering, promoters engineering and morphological engineering are summarized in detail. Finally, perspectives for future engineering approaches are proposed to improve β-carotene production.
ISSN:0738-8551
1549-7801
1549-7801
DOI:10.1080/07388551.2023.2166809