Ratiometric Near-Infrared Fluorescence Liposome Nanoprobe for H 2 S Detection In Vivo

• Published in: Molecules (Basel, Switzerland) Switzerland), 2023-02, Vol.28 (4)
• Main Authors: Wu, Luyan, Liu, Yili, Zhang, Junya, Miao, Yinxing, An, Ruibing
• Format: Article
• Language: English
• Subjects: Diffusion; Fluorescence; Fluorescent Dyes; Humans; Hydrogen Sulfide; Liposomes
• ISSN: 1420-3049

Accurate detection of H S is crucial to understanding the occurrence and development of H S-related diseases. However, the accurate and sensitive detection of H S in vivo still faces great challenges due to the characteristics of H S diffusion and short half-life. Herein, we report a H S-activatable ratiometric near-infrared (NIR) fluorescence liposome nanoprobe HS-CG by the thin-film hydration method. HS-CG shows "always on" fluorescence signal at 816 nm and low fluorescence signal at 728 nm; the NIR fluorescence ratio between 728 and 816 nm (F /F ) is low. Upon reaction with H S, the fluorescence at 728 nm could be more rapidly turned on due to strong electrostatic interaction between enriched HS and positively charged 1,2-dihexadecanoyl-sn-glycero-3-phosphocholine (DPPC) doped in the liposome nanoprobe HS-CG, resulting in a large enhancement of F /F , which allows for sensitive visualization of the tumor H S levels in vivo. This study demonstrates that this strategy of electrostatic adsorption between HS and positively charged molecules provides a new way to enhance the reaction rate of the probe and H S, thus serving as an effective platform for improving the sensitivity of imaging.