TiO 2- MWCNT nanohybrid: Cytotoxicity, protein corona formation and cellular internalisation in RTG-2 fish cell line

Titanium dioxide nanoparticles-multiwalled carbon nanotubes (TiO MWCNT) nanohydrid has an enhanced photocatalytic activity across the visible light with promising applications in environmental remediation, solar energy devices and antimicrobial technologies. However, it is necessary to evaluate the...

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Published in:Aquatic toxicology 2023-04, Vol.257, p.106434
Main Authors: Da Silva, Gabriela H, Franqui, Lidiane Silva, De Farias, Marcelo A, De Castro, Vera Lucia S S, Byrne, Hugh J, Martinez, Diego S T, Monteiro, Regina T R, Casey, Alan
Format: Article
Language:English
Subjects:
Animals
Cell Line
Fishes
Nanoparticles - toxicity
Nanotubes, Carbon - chemistry
Nanotubes, Carbon - toxicity
Protein Corona - chemistry
Titanium - chemistry
Titanium - toxicity
Water Pollutants, Chemical - toxicity
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Summary:Titanium dioxide nanoparticles-multiwalled carbon nanotubes (TiO MWCNT) nanohydrid has an enhanced photocatalytic activity across the visible light with promising applications in environmental remediation, solar energy devices and antimicrobial technologies. However, it is necessary to evaluate the toxicological effects of TiO MWCNT towards safe and sustainable development of nanohybrids. In this work, we studied the cytotoxicity, protein corona formation and cellular internalisation of TiO MWCNT on fibroblasts derived from gonadal rainbow trout tissue (RTG-2) for the first time. This nanohydrid did not show any toxicity effect on RTG-2 cells up to 100 mg L after 24 h of exposure as monitored by alamar blue, neutral red and trypan blue assays (in presence or absence of foetal bovine serum, FBS). Futhermore, cryo-transmission electron microscopy analysis demonstrated that TiO particles is attached on nanotube surface after FBS-protein corona formation in cell culture medium. Raman spectroscopy imaging showed that TiO MWCNT can be internalised by RTG-2 cells. This work is a novel contribution towards better understanding the nanobiointeractions of nanohydrids linked to their in vitro effects on fish cells in aquatic nanoecotoxicology.
ISSN:1879-1514