Effects of Two Calcium Silicate Cements on Transforming Growth Factor- β 1 Secretion from Human Dental Pulp Stem Cells

The aims of this study were to evaluate the effects of two calcium silicate based cements, Calcum-enriched Mixture (CEM) and Biodentine on proliferation of human dental pulp stem cells (hDPSCs) and the effects of proposed cements on the secretion of Transforming Growth Factor 1 (TGF- 1). The cell cu...

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Bibliographic Details
Published in:Iranian endodontic journal 2018, Vol.13 (4), p.522
Main Authors: Omidi, Salma, Bagheri, Mehdi, Fazli, Mozhgan, Ahmadiankia, Naghmeh
Format: Article
Language:English
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Summary:The aims of this study were to evaluate the effects of two calcium silicate based cements, Calcum-enriched Mixture (CEM) and Biodentine on proliferation of human dental pulp stem cells (hDPSCs) and the effects of proposed cements on the secretion of Transforming Growth Factor 1 (TGF- 1). The cell cultures of human Dental Pulp Stem Cells (hDPSCs) at passage 3-5 were treated with various dilutions (1/1, 1/2, 1/4, 1/8, 1/16, and 1/32) of CEM and Biodentine extracts to assess the cell proliferation using 3-(4, 5-dimethylthiazol-2-Y1)-2, 5-diphenyltetrazolium brovide (MTT) assay after 48 and 72 h. The amount of TGF- 1 secretion were estimated after 72 h using an enzyme-linked immunosorbent assay. Data were analyzed using the one-way analysis of variance (ANOVA) followed by the Dunnett's test at the level of significance set at 0.05. CEM showed the highest rates of cell proliferation compared to Biodentine after 72 h (
ISSN:2008-2746
DOI:10.22037/iej.v13i4.21885