Investigating the robustness of a deep learning-based method for quantitative phase retrieval from propagation-based x-ray phase contrast measurements under laboratory conditions

• Published in: Physics in medicine & biology 2023-04, Vol.68 (8), p.85005
• Main Authors: Deshpande, Rucha, Avachat, Ashish, Brooks, Frank J, Anastasio, Mark A
• Format: Article
• Language: English
• Subjects: Deep Learning; deep learning-based method; laboratory conditions; Microscopy, Phase-Contrast; propagation-based x-ray phase contrast imaging; quantitative phase retrieval; Radiography; X-Rays
• ISSN: 0031-9155; 1361-6560
• DOI: 10.1088/1361-6560/acc2aa

Quantitative phase retrieval (QPR) in propagation-based x-ray phase contrast imaging of heterogeneous and structurally complicated objects is challenging under laboratory conditions due to partial spatial coherence and polychromaticity. A deep learning-based method (DLBM) provides a nonlinear approach to this problem while not being constrained by restrictive assumptions about object properties and beam coherence. The objective of this work is to assess a DLBM for its applicability under practical scenarios by evaluating its robustness and generalizability under typical experimental variations. Towards this end, an end-to-end DLBM was employed for QPR under laboratory conditions and its robustness was investigated across various system and object conditions. The robustness of the method was tested via varying propagation distances and its generalizability with respect to object structure and experimental data was also tested. Although the end-to-end DLBM was stable under the studied variations, its successful deployment was found to be affected by choices pertaining to data pre-processing, network training considerations and system modeling. To our knowledge, we demonstrated for the first time, the potential applicability of an end-to-end learning-based QPR method, trained on simulated data, to experimental propagation-based x-ray phase contrast measurements acquired under laboratory conditions with a commercial x-ray source and a conventional detector. We considered conditions of polychromaticity, partial spatial coherence, and high noise levels, typical to laboratory conditions. This work further explored the robustness of this method to practical variations in propagation distances and object structure with the goal of assessing its potential for experimental use. Such an exploration of any DLBM (irrespective of its network architecture) before practical deployment provides an understanding of its potential behavior under experimental settings.