S(-II) reactivates Cd 2+ -stressed Shewanella oneidensis via promoting low-molecular-weight thiols synthesis and activating antioxidant defense

Efficient remedies for living organisms including bacteria to counteract cadmium (Cd ) toxicity are still highly needed. Plant toxicity studies have showed that exogenous S(-II) (including hydrogen sulfide and its ionic forms, i.e., H S, HS , and S ) application can effectively alleviate adverse eff...

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Published in:Environmental pollution (1987) 2023-06, Vol.327, p.121516
Main Authors: Zhang, Siyu, Ke, Changdong, Jiang, Mengge, Li, Yuancheng, Huang, Weilin, Dang, Zhi, Guo, Chuling
Format: Article
Language:English
Subjects:
Antioxidants - metabolism
Cadmium - toxicity
Ferric Compounds - metabolism
Oxidation-Reduction
Proteomics
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Summary:Efficient remedies for living organisms including bacteria to counteract cadmium (Cd ) toxicity are still highly needed. Plant toxicity studies have showed that exogenous S(-II) (including hydrogen sulfide and its ionic forms, i.e., H S, HS , and S ) application can effectively alleviate adverse effects of Cd stress, but whether S(-II) could mitigate bacterial Cd toxicity remains unclear. In this study, S(-II) was applied exogenously to Cd-stressed Shewanella oneidensis MR-1 and the results showed that S(-II) can significantly reactivate impaired physiological processes including growth arrest and enzymatic ferric (Fe(III) reduction inhibition. The efficacy of S(-II) treatment is negatively correlated with the concentration and time length of Cd exposure. Energy-dispersive X-ray (EDX) analysis suggested the presence of cadmium sulfide inside cells treated with S(-II). Both compared proteomic analysis and RT-qPCR showed that enzymes associated with sulfate transport, sulfur assimilation, methionine, and glutathione biosynthesis were up-regulated in both mRNA and protein levels after the treatment, indicating S(-II) may induce the biosynthesis of functional low-molecular-weight (LMW) thiols to counteract Cd toxicity. Meanwhile, the antioxidant enzymes were positively modulated by S(-II) and thus the activity of intracellular reactive oxygen species was attenuated. The study demonstrated that exogenous S(-II) can effectively alleviate Cd stress for S. oneidensis likely through inducing intracellular trapping mechanisms and modulating cellular redox status. It suggested that S(-II) may be a highly effective remedy for bacteria such as S. oneidensis under Cd-polluted environments.
ISSN:1873-6424