A methionine biomolecule-modified chromenylium-cyanine fluorescent probe for the analysis of Hg 2+ in the environment and living cells

The objective of the study is, for the first time, to construct a new near infrared (NIR) fluorophore, spectrophotometric, colorimetric, ratiometric, and turn-on probe (CSME) based on chromenylium cyanine platform decorated with methionine biomolecule to provide an efficient solution for critical sh...

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Bibliographic Details
Published in:Talanta (Oxford) 2023-07, Vol.259, p.124471
Main Authors: Alcay, Yusuf, Ozdemir, Emre, Yildirim, Mustafa Semih, Ertugral, Utku, Yavuz, Ozgur, Aribuga, Hulya, Ozkilic, Yilmaz, Şenyurt Tuzun, Nurcan, Ozdabak Sert, Ayse Buse, Kok, Fatma Nese, Yilmaz, Ismail
Format: Article
Language:English
Subjects:
Animals
Fluorescent Dyes - chemistry
Mercury - analysis
Methionine
Mice
Racemethionine
Water - chemistry
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Summary:The objective of the study is, for the first time, to construct a new near infrared (NIR) fluorophore, spectrophotometric, colorimetric, ratiometric, and turn-on probe (CSME) based on chromenylium cyanine platform decorated with methionine biomolecule to provide an efficient solution for critical shortcoming to be encountered for analysis of hazardous Hg in environment and living cell. The CSME structure and its interaction with Hg ion were evaluated by NMR, FTIR, MS, UV-Vis and fluorescence methods as well as Density Functional Theory (DFT) calculations. The none fluorescence CSME having spirolactam ring only interacted with Hg in aqueous solution including competing ions. This interaction caused the fluorescence CSME with opened spirolactam form which exhibited spectral and colorimetric changes in the NIR region. The probe based on UV-Vis and fluorescence techniques respond in 90 s, has wide linear ranges (for UV-Vis: 6.29 × 10 - 1.86 × 10  M; for fluorescence: 9.49 × 10 - 1.13 × 10  M), and has a lower Limit of Detection (LOD) value (for fluorescence: 4.93 × 10  M, 0.99 ng/mL) than the value predicted by the US Environmental Protection Agency (EPA) organization. Hg analysis was performed in drinking and tap water with low Relative Standard Deviation (RSD) values and high recovery. Smartphone and living cell applications were successfully performed for colorimetric sensing Hg in real samples and 3T3 cells, respectively.
ISSN:1873-3573