Antibacterial and Anti-Biofilm Activities of Microbial Synthesized Silver and Magnetic Iron Oxide Nanoparticles against Pseudomonas aeruginosa

• Published in: IEEE transactions on nanobioscience 2023-10, Vol.PP (4), p.1-1
• Main Authors: Esfahani, Mahbobeh Baghiat, Khodavandi, Alireza, Alizadeh, Fahimeh, Bahador, Nima
• Format: Magazinearticle
• Language: English
• Subjects: <italic xmlns:ali="http://www.niso.org/schemas/ali/1.0/" xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance">PELA; <italic xmlns:ali="http://www.niso.org/schemas/ali/1.0/" xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance">PSLA; Antibacterial activity; Biofilm-associated genes; Biofilms; Ceftazidime; Ceftazidime resistance; Clinical isolates; Effectiveness; Extracellular; Gene expression; Genes; Gentian violet; Immune system; Iron oxides; Light microscopy; Microbial synthesized nanoparticles; Microorganisms; Nanobioscience; Nanoparticles; Optical microscopy; Pseudomonas aeruginosa; Silver; Synthesis; Virulence factors
• ISSN: 1536-1241; 1558-2639
• DOI: 10.1109/TNB.2023.3268138

Pseudomonas aeruginosa is a human bacterial pathogen causing devastating diseases and equipped with various virulence factors like biofilm formation. Common antibiotic treatment has limited efficacy for the P. aeruginosa present in biofilms because of the increased resistance. In this study, we focused our attention on the antibacterial and anti-biofilm activities of various microbial synthesized silver (nano-Ag) and magnetic iron oxide (nano-Fe 3 O 4 ) nanoparticles against clinical isolates of P. aeruginosa that displayed ceftazidime resistance. The nano-Ag and nano-Fe 3 O 4 represented great antibacterial properties. Nano-Ag and nano-Fe 3 O 4 exhibited a reduction in the biofilm formation by P. aeruginosa reference strain as determined by crystal violet and XTT assays and light microscopy method. Among all, nano-Ag-2 and 7 owing to inherent attributes and mechanisms of resistance in the bacterial biofilm, exhibited anti-biofilm efficacy against ceftazidime resistance clinical isolate of P. aeruginosa . Moreover, nano-Ag and nano-Fe 3 O 4 changed the relative expression of biofilm-associated genes, PELA and PSLA in a concentration dependent manner by P. aeruginosa reference strain. As revealed by qRT-PCR, the expression levels of biofilm-associated genes were downregulated in P. aeruginosa biofilms treated with nano-Ag, while selected biofilm-associated genes were low expressed under treated with nano-Fe 3 O 4 . Results of the study demonstrate the potential of microbial synthesized nano-Ag-2 and 7 to act as anti-biofilm agents against ceftazidime resistance clinical isolate of P. aeruginosa . Molecular targeting of biofilm-associated genes by nano-Ag and nano-Fe 3 O 4 may be candidate for new therapeutics against P. aeruginosa diseases.