In situ synthesized eRAFT polymers for highly sensitive electrochemical determination of AFB 1 in foods and herbs

An electrochemical sensor based on environmentally friendly eRAFT polymerization was developed for the detection of aflatoxin B (AFB ) in food and herbal medicine. Two biological probes, aptamer (Ap) and antibody (Ab), were used to specifically recognize AFB , and a large number of ferrocene polymer...

Full description

Saved in:
Bibliographic Details
Published in:Food chemistry 2023-09, Vol.421, p.136176
Main Authors: Zhou, Shijin, Guo, Liang, Shi, Xinheng, Ma, Lele, Yang, Huaixia, Miao, Mingsan
Format: Article
Language:English
Subjects:
Aflatoxin B1 - analysis
Aptamers, Nucleotide
Biosensing Techniques - methods
Electrochemical Techniques - methods
Limit of Detection
Polymers
Reproducibility of Results
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:An electrochemical sensor based on environmentally friendly eRAFT polymerization was developed for the detection of aflatoxin B (AFB ) in food and herbal medicine. Two biological probes, aptamer (Ap) and antibody (Ab), were used to specifically recognize AFB , and a large number of ferrocene polymers were grafted on the electrode surface by eRAFT polymerization, which greatly improved the specificity and sensitivity of the sensor. The detection limit of AFB was 37.34 fg/mL. In addition, the recovery rate was 95.69% to 107.65% and the RSD was 0.84% to 4.92% by detecting 9 spiked samples. The delighted reliability of this method was verified by HPLC-FL.
ISSN:1873-7072