Recognition of the interaction between the bioactive peptide Val-Pro-Pro and the minimal promoter region of genes SOD and CAT using QCM-D and docking studies

Bioactive peptides are biomolecules involved in very diverse mechanisms in vivo . It has been reported that bioactive peptides play a very important role in the regulation of physiological functions such as oxidative stress, hypertension, cancer and inflammation. It's been reported that the mil...

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Published in:Analytical methods 2023-06, Vol.15 (24), p.2979-2988
Main Authors: Pérez-Vielma, Nadia Mabel, Gómez-López, Modesto, Maldonado, Jesús, Correa-Basurto, José, Martínez-Godínez, María de los Ángeles, Miliar-García, Ángel
Format: Article
Language:English
Subjects:
Adipose tissue
Animal models
Animal tissues
Biological activity
Biomolecules
Biosensors
Catalase
Deoxyribonucleic acid
DNA
Energy value
Free energy
Genes
Hydrogen bonding
Hydrogen bonds
Hypertension
In vivo methods and tests
Inflammation
Molecular docking
Molecular modelling
Oral administration
Oxidation
Oxidative stress
Peptides
Piezoelectricity
Quartz crystal microbalance
Superoxide dismutase
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Summary:Bioactive peptides are biomolecules involved in very diverse mechanisms in vivo . It has been reported that bioactive peptides play a very important role in the regulation of physiological functions such as oxidative stress, hypertension, cancer and inflammation. It's been reported that the milk derived peptide (VPP) prevents the progress of hypertension in different animal models and human beings with mild hypertension. It has also been shown that oral administration of VPP produces an anti-inflammatory effect in adipose tissue of mouse models. Currently there are no reports on the possible interaction of VPP with the enzymes superoxide dismutase (SOD) and catalase (CAT), the main regulators of oxidative stress. This study analyzes the interaction between VPP and specific domains in the minimal promoter region of the genes SOD and CAT in blood samples of obese children using a QCM-D type piezoelectric biosensor. We also used molecular modeling (docking) to determine the interaction between the peptide VPP and the minimal promoter region of both genes. With QCM-D, we detected the interaction of VPP with the nitrogenous base sequences that comprise the minimal promoter regions of both genes CAT and SOD. These experimental interactions were explained at the atomic level by molecular docking simulations showing how the peptides are capable of reaching the DNA structures by means of hydrogen bonds with favored free energy values. It is possible to conclude that the combined use of docking and QCM-D allows for the determination of the interaction of small peptides (VPP) with specific sequences of genes. Recognition of interaction of small peptides (VPP) with specific sequences of genes.
ISSN:1759-9660
1759-9679
DOI:10.1039/d3ay00265a