Effect of cancer-related and drug-induced alterations in surface carbohydrates on the invasive capacity of mouse and rat cells

The effect of alterations in cell surface carbohydrates on invasion of mouse and rat cells into embryonic chick heart fragments in organ culture was studied. Matching pairs of malignant and nonmalignant cells, including all categories of carcinogenic induction (i.e., viral, chemical, or oncogenic),...

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Bibliographic Details
Published in:Cancer research (Chicago, Ill.) Ill.), 1986-08, Vol.46 (8), p.4080-4086
Main Authors: BOLSCHER, J. G. M, SCHALLIER, D. C. C, SMETS, L. A, VAN ROOY, H, COLLARD, J. G, BRUYNEEL, E. A, MAREEL, M. M. K
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Carbohydrates - analysis
Cell Adhesion
Cell Aggregation
Cell Communication
Cell Membrane - analysis
Cell Transformation, Neoplastic - metabolism
Chick Embryo
Fucose - metabolism
General aspects (metabolism, cell proliferation, established cell line...)
Glycopeptides - analysis
Lysophospholipids
Medical sciences
Mice
Neoplasm Invasiveness
Oncogenes
Organ Culture Techniques
Phospholipids - pharmacology
Rats
Tumor cell
Tumors
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Summary:The effect of alterations in cell surface carbohydrates on invasion of mouse and rat cells into embryonic chick heart fragments in organ culture was studied. Matching pairs of malignant and nonmalignant cells, including all categories of carcinogenic induction (i.e., viral, chemical, or oncogenic), were compared for their alterations in cell surface carbohydrates and invasive behavior. Glycopeptides derived from the surface of malignant cells expressed cancer-related changes in carbohydrate composition, demonstrated by gel filtration chromatography as a shift in size distribution in comparison with those from nonmalignant counterparts. This phenotypic property strictly correlated with the acquisition of the invasive capacity. Morphological transformation of cells without simultaneous alteration in surface carbohydrates was, however, insufficient for invasion. To test the possible mechanistic role of altered surface carbohydrates in the invasive capacity of cells, the surface molecules of noninvasive cells were modified by incubation with an alkyl-lysophospholipid (racemic 1-O-octadecyl-2-O-methyl glycero-3-phosphocholine). Alkyl-lysophospholipid induced an increase in surface sialylation resembling the changes found in malignant and invasive cells. After pretreatment with alkyl-lysophospholipid, morphologically transformed but nonmalignant and noninvasive cells became able to invade chick heart tissue. These findings indicate that alterations in cell surface carbohydrates, induced by entirely different mechanisms, endowed cells with invasive capacity.
ISSN:0008-5472
1538-7445