Characterization and the cholesterol-lowering effect of dietary fiber from fermented black rice ( L.)

• Published in: Food & function 2023-07, Vol.14 (13), p.6128-6141
• Main Authors: Guan, Yuting, Xie, Chanyuan, Zhang, Rui, Zhang, Ziyang, Tian, Zhenyang, Feng, Jianing, Shen, Xiaoyong, Li, Haiqin, Chang, Shimin, Zhao, Changhui, Chai, Ran
• Format: Article
• Language: English
• Subjects: Adsorption; Body weight; Cholesterol; Dietary fiber; Fatty acids; Fermentation; Fermented food; Intestinal microflora; Lipid metabolism; Lipids; Metabolism; Neurospora; Rice; Sodium cholate; Structure-function relationships; Weight reduction
• ISSN: 2042-6496; 2042-650X
• DOI: 10.1039/d3fo01308a

Black rice was fermented with Neurospora crassa , after which the dietary fiber (DF) extracted from it was characterized and evaluated for its cholesterol-lowering effect in mice. The findings demonstrated that fermentation increased the level of soluble DF from 17.27% ± 0.12 to 29.69% ± 0.26 and increased the adsorption capacity of DF for water, oil, cholesterol, glucose and sodium cholate. The fermented DF had a more loose and porous structure than that extracted from unfermented rice. Additionally, feeding with DF from the fermented black rice significantly reduced body weight, lowered total cholesterol levels and improved the lipid profile in mice gavaged with a high dose (5 g per kg bw) or a low dose (2.5 g per kg·bw). ELISA showed that the hepatic expression of typical proteins and enzymes that are involved in cholesterol metabolism was regulated by the fermented rice DF, leading to reduced cholesterol production and increased cholesterol clearance. The fermented DF also modified the gut microbiota composition ( e.g. Firmicutes reduced and Akkermansia increased), which promoted the production of short-chain fatty acids. In conclusion, fermentation can modify the structure and function of DF in black rice and the fermented dietary fiber has excellent cholesterol lowering effects possibly by cholesterol adsorption, cholesterol metabolism modulation, and intestinal microflora regulation. The dietary fiber extracted from the fermented black rice showed a loose/porous structure and high adsorption capacity. The fermented dietary fiber improved the lipid profile and modified the gut microbiota in hypercholesterolemic mice.