A novel multimode biosensor for sensitive detection of AFB 1 in food based on Mxenes nano enzymes

In this work, Ti C nano-enzymes (Ti C NEs) materials with simulated peroxidase activity and fluorescence quenching properties were prepared. Then Ti C NEs was functionalized using 6-carboxyfluorescein (FAM) labeled Aflatoxin B (AFB ) aptamers to construct a novel multimode nano enzyme biosensor for...

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Bibliographic Details
Published in:Food chemistry 2023-11, Vol.426, p.136645
Main Authors: Kong, Yiqian, Zhu, Yinghua, Song, Juncheng, Liu, Qi, Song, Lili, Fei, Xiaowei, Li, Xiangyang
Format: Article
Language:English
Subjects:
Aflatoxin B1 - analysis
Aptamers, Nucleotide - chemistry
Arachis
Biosensing Techniques - methods
Food
Food Contamination - analysis
Limit of Detection
Peroxidases
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Summary:In this work, Ti C nano-enzymes (Ti C NEs) materials with simulated peroxidase activity and fluorescence quenching properties were prepared. Then Ti C NEs was functionalized using 6-carboxyfluorescein (FAM) labeled Aflatoxin B (AFB ) aptamers to construct a novel multimode nano enzyme biosensor for the detection of AFB in peanuts. Based on the fluorescence quenching characteristics and the superior simulated peroxidase activity of Ti C NE and the specific binding of the aptamer to AFB , the sensitive and rapid fluorescence/colorimetric/smart phone detection of AFB have been achieved, with detection limits of 0.09 ng mL , 0.61 ng mL and 0.96 ng mL , respectively. The analytical method provided can not only detect AFB in multiple modes, but also has a wider detection range, lower limit of detection (LOD) and better recovery rate, and can achieve on-site accurate detection of AFB content in peanuts, which has great application potential in the field of food quality testing.
ISSN:1873-7072