Ratiometric fluorescence immunoassay based on silver nanoclusters and calcein-Ce 3+ for detecting ochratoxin A

Ochratoxin A (OTA), a dangerous mycotoxin, is found in many crops. It is essential to create sensitive OTA detection techniques to ensure food safety. Based on the principle of p-nitrophenol (PNP) quenched the fluorescence of bovine serum albumin silver nanocluster (BSA-AgNCs) through an internal fi...

Full description

Saved in:
Bibliographic Details
Published in:Talanta (Oxford) 2024-03, Vol.269, p.125470
Main Authors: Zhao, Yanan, Chen, Wang, Fang, Huajuan, Zhang, Junxiang, Wu, Shixiang, Yang, Hualin, Zhou, Yu
Format: Article
Language:English
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Ochratoxin A (OTA), a dangerous mycotoxin, is found in many crops. It is essential to create sensitive OTA detection techniques to ensure food safety. Based on the principle of p-nitrophenol (PNP) quenched the fluorescence of bovine serum albumin silver nanocluster (BSA-AgNCs) through an internal filtering effect, and phosphate activated fluorescence of calcein-Ce system, a ratiometric fluorescence immunoassay for OTA detection was developed. In this strategy, the value of F /F was used as a signal for response of OTA concentration. The detection range of this strategy was 0.625-25 ng/mL, the limit of detection (LOD) was 0.04 ng/mL. This new immunoassay offered a brand-new platform for detecting OTA.
ISSN:1873-3573