Localization and expression of phospholipase A 2 and polyunsaturated fatty acid profile in the testis tissues of Hu sheep

The fatty acid content and the localization and expression of phospholipase A (PLA ) in the testis of Hu sheep were investigated. A total of 18 six-month-old Hu sheep were divided into small group (S, with left testis weight < 50 g), medium group (M, with left testis weight among 90-110 g), and l...

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Bibliographic Details
Published in:Animal reproduction science 2024-01, Vol.260, p.107381
Main Authors: Liang, Weili, Zhao, Yanhong, Quan, Guodong, Yao, Rongyu, Chen, Hua, Weng, Xiuxiu, Li, Wanhong, Yue, Xiangpeng, Li, Fadi
Format: Article
Language:English
Subjects:
Animals
Fatty Acids, Omega-3
Fatty Acids, Unsaturated
Humans
Male
Phospholipases A2 - genetics
Sertoli Cells
Sheep
Testis
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Summary:The fatty acid content and the localization and expression of phospholipase A (PLA ) in the testis of Hu sheep were investigated. A total of 18 six-month-old Hu sheep were divided into small group (S, with left testis weight < 50 g), medium group (M, with left testis weight among 90-110 g), and large group (L, with left testis weight >160 g), which had six individuals each. The expression of PLA in testicular tissues of different sizes was analyzed by immunohistochemistry, RT-qPCR, and Western blot. The fatty acid profile was detected by gas chromatography. Immunohistochemical labeling determined that PLA protein was expressed in the Leydig and Sertoli cells of testis, and the immunohistochemical average optional density in the S group was significantly greater than the L group (P 
ISSN:1873-2232
DOI:10.1016/j.anireprosci.2023.107381