Quantifying energy expenditure in Göttingen Minipigs with the 13 C-bicarbonate method under basal and drug-treated conditions

Effective treatments of obesity focusing on energy expenditure (EE) are needed. To evaluate future EE-modulating drug candidates, appropriate animal models and methods to assess EE are needed. This study aimed to evaluate the stable isotope C-bicarbonate method ( C-BM) for estimating EE in Göttingen...

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Bibliographic Details
Published in:Clinical nutrition ESPEN 2023-12, Vol.58, p.388
Main Authors: Bredum, Simon K, Strathe, Anja V, Jacobsen, Julie, Christoffersen, Berit Ø, Gehre, Matthias, Kümmel, Steffen, Junghans, Peter, Marcussen, Caroline
Format: Article
Language:English
Subjects:
Animals
Bicarbonates
Cross-Over Studies
Energy Metabolism
Isotopes
Pharmaceutical Preparations
Reproducibility of Results
Swine
Swine, Miniature
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Summary:Effective treatments of obesity focusing on energy expenditure (EE) are needed. To evaluate future EE-modulating drug candidates, appropriate animal models and methods to assess EE are needed. This study aimed to evaluate the stable isotope C-bicarbonate method ( C-BM) for estimating EE in Göttingen minipigs under basal and drug-treated conditions. Four experiments (Expt.1-4) were conducted to assess: 1) the C-BM reproducibility using breath sample collection (n = 8), on two consecutive days, 2) the effect of two dose levels (5 and 10 mg/kg body weight (BW)) of the mitochondrial uncoupler dinitrophenol (DNP) in a crossover design (n = 8), 3) sampling method agreement; blood vs. exhaled air (n = 6) and 4) C-BM using constant isotope infusion compared with indirect calorimetry (IC) (n = 3). Results correlated significantly (p 
ISSN:2405-4577